H103 Cell Line

Cat. #153419

H103 Cell Line

Cat. #: 153419

Unit size: 1x10^6 cells / vial

Tissue: Tongue

Disease: Cancer

Model: Tumour line


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Inventor: Stephen Prime

Institute: University of Bristol

Tool Details
Target Details

Tool Details


  • Name: H103 Cell Line
  • Research fields: Cancer
  • Tissue: Tongue
  • Disease: Cancer
  • Growth properties: Adherent
  • Model: Tumour line
  • Description: Established from a small squamous cell carcinoma (SSC) of the tongue (<20mm in size) of a 32 year-old male patient. STNMP stage 1, well differentiated, node negative tumour. Aneuploid- dual G-/G1 peak on cell cycle analysis by flow cytometry. Mutant p53, codon 244 exon 7; G to T; wild type K-, N- and Ha-ras. Tumourigenic in athymic nude mice, both by subcutaneous injection and injection into the floor of the mouth. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
  • Cellosaurus id: CVCL_2457

Target Details

  • Target: Human oral squamous cell carcinoma, tongue


  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%) 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37?‚°C. Suggested seeding density 5 x 1000 cells/cm?‹Â› . Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydroco...
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice


  • Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
  • Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
  • Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893.
  • TGF-beta receptor regulation mediates the response to exogenous ligand but is independent of the degree of cellular differentiation in human oral keratinocytes.
  • Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.
  • Epidermal growth f...