#153421

H314 Cell Line

Cat. #153421

H314 Cell Line

Cat. #: 153421

Unit size: 1x10^6 cells / vial

Tissue: Oral Cavity

Disease: Cancer

Model: Tumour line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Stephen Prime

Institute: University of Bristol

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: H314 Cell Line
  • Research fields: Cancer
  • Tissue: Oral Cavity
  • Disease: Cancer
  • Growth properties: Adherent
  • Model: Tumour line
  • Conditional: Yes
  • Description: Established from a squamous cell carcinoma (SCC) of the floor of the mouth (20mm-40mm) of an 82 year-old male patient. STNMP stage II, moderately differentiated, node positive tumour. Mutant p53, codon 176 exon 5, G to T and 373 exon 11, A to G; wild type K-, N- and Ha-ras. Tumourigenic in athymic nude mice on subcutaneous injection; on injection into the floor of the mouth, cells spread to lymphatics and lymph nodes. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
  • Cellosaurus id: CVCL_2460

Target Details

  • Target: Human oral squamous cell carcinoma, floor of mouth

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%), approximately every 6-7 days, 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37?‚°C. Suggested seeding density 7 x 1000 cells/cm?‹Â›. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
  • Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
  • Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893.
  • TGF-beta receptor regulation mediates the response to exogenous ligand but is independent of the degree of cellular differentiation in human oral keratinocytes.
  • Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.
  • Epidermal growth f...