#151655

FLYA-13 Cell Line

Please Enter Your Email ID
Email ID

Cat. #151655

FLYA-13 Cell Line

Cat. #: 151655

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Organism: Human

Disease: Cancer

Model: Packaging

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Mary Collins

Institute: The Institute of Cancer Research London

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: FLYA-13 Cell Line
  • Cancer: Sarcoma
  • Cancers detailed: Fibrosarcoma
  • Research fields: Cancer;Genetics
  • Tool sub type: Continuous
  • Parental cell: HT 1080
  • Organism: Human
  • Disease: Cancer
  • Growth properties: Recombinant retroviral production
  • Model: Packaging
  • Conditional: Yes
  • Description: The FLYA-13 Cell Line is a packaging cell line enabling production of high-titer, human complement-resistant recombinant retroviruses, with significantly reduced probability of replication-competent retrovirus generation. HT 1080-based packaging cell line enabling production of recombinant retroviral vectors with Moloney murine leukemia virus cores and amphotropic murine leukemia virus envelopes. The vectors demonstrate high resistance to the inhibitory effects of human serum/complement, in...
  • Production details: For details of production of FLYA13 cell line see Cosset et al. 1995. Journal of Virology. 69:7430-36. PMID: 7494248.
  • Cellosaurus id: CVCL_8870

Handling

  • Format: Frozen
  • Growth medium: For recommended growth and recombinant retrovirus production conditions see Cosset F et al, Journal of Virology, 1995, v69 pp7430-7436 & Takeuchi Y et al, Journal of Virology, 1994, v68 pp8001-8007
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Cosset et al. 1995. J Virol. 69(12):7430-6. PMID: 7494248.
  • High-titer packaging cells producing recombinant retroviruses resistant to human serum.
  • Takeuchi et al. 1994. J Virol. 68(12):8001-7. PMID: 7966590.
  • Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell.