FLYA-13 Cell Line

FLYA-13 Cell Line is a packaging cell line enabling production of high-titer, human complement-resistant recombinant retroviruses

Contributors

Inventor Institute
Mary Collins The Institute of Cancer Research London
Cat. #: 151655
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Sarcoma
Research Fields: Cancer;Genetics
Organism: Human
Model: Packaging
Growth properties: Recombinant retroviral production
Cancer Types In Detail: Fibrosarcoma
Product description: The FLYA-13 Cell Line is a packaging cell line enabling production of high-titer, human complement-resistant recombinant retroviruses, with significantly reduced probability of replication-competent retrovirus generation. HT 1080-based packaging cell line enabling production of recombinant retroviral vectors with Moloney murine leukemia virus cores and amphotropic murine leukemia virus envelopes. The vectors demonstrate high resistance to the inhibitory effects of human serum/complement, in…
Conditional: Yes
Production details: For details of production of FLYA13 cell line see Cosset et al. 1995. Journal of Virology. 69:7430-36. PMID: 7494248.
Cellosaurus ID: CVCL_8870
Parental cell line: HT 1080
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculture routine: Split sub-confluent cultures (70-80%) 1:20 i.e. seeding at 1×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
References:

Cosset et al. 1995. J Virol. 69(12):7430-6. PMID: 7494248.

High-titer packaging cells producing recombinant retroviruses resistant to human serum.

Takeuchi et al. 1994. J Virol. 68(12):8001-7. PMID: 7966590.

Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell.

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