FLYA-13 Cell Line
Exclusive toolFLYA-13 Cell Line is a packaging cell line enabling production of high-titer, human complement-resistant recombinant retroviruses
Contributors
| Inventor | Institute |
|---|---|
| Mary Collins | The Institute of Cancer Research London |
| SKU: | 151655 |
|---|---|
| Product description: | FLYA-13 Cell Line is a packaging cell line enabling production of high-titer, human complement-resistant recombinant retroviruses |
| Conditional: | Yes |
| Production details: | For details of production of FLYA13 cell line see Cosset et al. 1995. Journal of Virology. 69:7430-36. PMID: 7494248. |
| Cellosaurus ID: | CVCL_8870 |
| Parental cell line: | HT 1080 |
| Disease: | Cancer |
| Cat. #: | 151655 |
|---|---|
| Provenance and Ownership: | The FLYA-13 Cell Line is owned by Cancer Research UK. CancerTools supplies the FLYA-13 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing FLYA-13 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Cancer; Genetics |
| Organism: | Human |
| Model: | Packaging |
| Growth properties: | Recombinant retroviral production |
| Cancer Types In Detail: | Fibrosarcoma |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) |
| Subculture routine: | Split sub-confluent cultures (70-80%) 1:20 i.e. seeding at 1×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. |
| References: |
Cosset et al. 1995. J Virol. 69(12):7430-6. PMID: 7494248. High-titer packaging cells producing recombinant retroviruses resistant to human serum. Takeuchi et al. 1994. J Virol. 68(12):8001-7. PMID: 7966590. Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell. |
|---|
Powered by Bioz 