H157 Cell Line

H157 Cell Line – Established from a squamous cell carcinoma (SCC) of the buccal mucosa (20mm-40mm) of a male patient, age 84.

Contributors

Inventor Institute
Stephen Prime University of Bristol
Cat. #: 153420
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Cancer types: Head and neck cancer;Head and neck cancer
Research Fields: Cancer;Cancer
Organism: Canine
Tissue: Buccal mucosa
Gender: Male
Model: Tumour cell line
Growth properties: Adherent
Cancer Types In Detail: Head and neck cancer;Head and neck cancer
Alternate name: H-157; h157
Product description: H157 was established from a squamous cell carcinoma (SCC) of the buccal mucosa (20mm-40mm) of a male patient, age 84. H157 has the following features: polygonal morphology, STNMP stage II, well differentiated and node positive tumour. This cell line has mutant p53, codon 306 exon 8, G to A; wild type K-, N- and Ha-ras. Cultured cells are keratin-positive. H157 cells are non-tumourigenic in athymic nude mice by subcutaneous injection and on injection into the floor of the mouth (orthotopic), but form epidermoid cysts subcutaneously. Explore more human oral SCC cell lines from the same inventor including: H357, H376, H400, H314 and H103.
Gender: Male
CRISPR: Yes
Conditional: Yes
Cellosaurus ID: CVCL_2458
disease: Cancer;Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
Subculture routine: Split sub-confluent cultures (70-80%) 1:8 to 1:10. Recommended growth conditions, 5% CO₂; 37°C. Suggested seeding density of 5 x 1000 cells/cm².
Mycoplasma free: Yes
References:

Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31(2):136-143. PMID: 7633286.

Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.

Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-67. PMID: 8180579.

Prime et al. 1990. J Pathol. 160(3):259-269. PMID: 1692339.

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