#153420

H157 Cell Line

Cat. #153420

H157 Cell Line

Cat. #: 153420

Unit size: 1x10^6 cells / vial

Tissue: Buccal mucosa

Disease: Cancer

Model: Tumour line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Stephen Prime

Institute: University of Bristol

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: H157 Cell Line
  • Research fields: Cancer
  • Tissue: Buccal mucosa
  • Disease: Cancer
  • Growth properties: Adherent
  • Model: Tumour line
  • Conditional: Yes
  • Description: Established from a squamous cell carcinoma (SCC) of the buccal mucosa (20mm-40mm) of a male patient, age 84. STNMP stage II, well differentiated, node positive tumour. Mutant p53, codon 306 exon 8, G to A; wild type K-, N- and Ha-ras. Non-tumourigenic in athymic nude mice by subcutaneous injection and on injection into the floor of the mouth (orthotopic), but form epidermoid cysts subcutaneously
  • Cellosaurus id: CVCL_2458

Target Details

  • Target: Human oral squamous cell carcinoma

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%) 1:8 to 1:10 5% CO2; 37?‚°C. Suggested seeding density 5 x 1000 cells/cm?‹Â›. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
  • Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
  • Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.
  • Epidermal growth factor and transforming growth factor alpha characteristics of human oral carcinoma cell lines.
  • Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-7. PMID: 8180579.
  • Ras gene point mutation is a rare event in premalign...