PDVC57B Cell Line

The PDVC57B Cell Line is useful for the study of the progression of carcinogenesis and as a model of relapse after surgery. It is syngeneic for C57B1/6 mice

Contributors

Inventor Institute
Allan Balmain Cancer Research UK, Glasgow: The Beatson Institute
Cat. #: 151630
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Carcinoma;Carcinoma
Organism: Mouse
Gender: Male
Model: Tumour line
Growth properties: Mouse spindle cell carcinoma, Adherent
Cancer Types In Detail: Carcinoma Squamous Cell;Carcinoma Squamous Cell
Product description: The PDVC57B Cell Line is useful for the study of the progression of carcinogenesis and as a model of relapse after surgery. PDVC57B is syngeneic for C57B1/6 mice and carries a p53 mutation in codon 231, (ATG-GTC) and an A-T tranversion at codon 61 of the H-ras gene. These events appear to be remarkably similar to the sequence of changes seen during tumour development in vivo.
Gender: Male
CRISPR: Yes
Conditional: Yes
Production details: The transformed PDVB57B was created by treating the PDV cell line with the carcinogen DMBA.
Cellosaurus ID: CVCL_4271
Parental cell line: PDV
disease: Cancer;Cancer
Format: Frozen
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry ice
Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture routine: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-5×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C
Mycoplasma free: Yes
References:

Oakley et al. 2002. Clin Cancer Res. 8(6):1984-1994. PMID: 12060644.

Quintanilla et al. 1991. Carcinogenesis. 12(10):1875-1881. PMID: 1934268.

Citations And Reviews

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