FLYRD18 Cell Line

The FLYRD18 Packaging cell line enables production of high-titer, human complement-resistant recombinant retroviruses, with significantly reduced probability of replication-competent retrovirus generation.

Contributors

Inventor Institute
Mary Collins The Institute of Cancer Research
Cat. #: 151446
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Sarcoma
Research Fields: Genetics;Genetics
Organism: Human
Gender: Male
Model: Packaging
Growth properties: Recombinant retroviral production
Cancer Types In Detail: Fibrosarcoma;Fibrosarcoma
Product description: The FLYRD18 Packaging cell line enables production of high-titer, human complement-resistant recombinant retroviruses, with significantly reduced probability of replication-competent retrovirus generation. HT1080-based packaging cell line enabling production of recombinant retroviral vectors with Moloney murine leukemia virus cores and cat endogenous virus RD114 virus envelopes. The vectors demonstrate high resistance to the inhibitory effects of human serum/complement, increasing penetration of the vector, and making the system ideal for in vivo gene transfer.
Gender: Male
CRISPR: Yes
Conditional: Yes
Production details: Human; for details of production of FLYRD18 cell line see Cosset et al. 1995. Journal of Virology. 69:7430-36. PMID: 7494248
Cellosaurus ID: CVCL_8871
Parental cell line: HT 1080
disease: Cancer;Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture routine: Split sub-confluent cultures (70-80%) 1:20 i.e. seeding at 1×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Mycoplasma free: Yes
References:

Production of retroviral vectors for gene therapy with the human packaging cell line FLYRD18.

Cosset et al. 1995. J Virol. 69(12):7430-6. PMID: 7494248.

High-titer packaging cells producing recombinant retroviruses resistant to human serum.

Takeuchi et al. 1994. J Virol. 68(12):8001-7. PMID: 7966590.

Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell.

Images

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FLYRD18 Cell Line. 24 hours post plating. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC).

Citations And Reviews

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