#151446

FLYRD18 Cell Line

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Cat. #151446

FLYRD18 Cell Line

Cat. #: 151446

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Organism: Human

Disease: Cancer

Model: Packaging

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Mary Collins

Institute: The Institute of Cancer Research

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: FLYRD18 Cell Line
  • Cancer: Sarcoma
  • Cancers detailed: Fibrosarcoma
  • Research fields: Genetics
  • Tool sub type: Continuous
  • Parental cell: HT 1080
  • Organism: Human
  • Disease: Cancer
  • Growth properties: Recombinant retroviral production
  • Model: Packaging
  • Conditional: Yes
  • Description: The FLYRD18 Packaging cell line enables production of high-titer, human complement-resistant recombinant retroviruses, with significantly reduced probability of replication-competent retrovirus generation. HT1080-based packaging cell line enabling production of recombinant retroviral vectors with Moloney murine leukemia virus cores and cat endogenous virus RD114 virus envelopes. The vectors demonstrate high resistance to the inhibitory effects of human serum/complement, increasing penetration of the vector, and making the system ideal for in vivo gene transfer.
  • Production details: Human; for details of production of FLYRD18 cell line see Cosset et al. 1995. Journal of Virology. 69:7430-36. PMID: 7494248
  • Cellosaurus id: CVCL_8871

Handling

  • Format: Frozen
  • Growth medium: For recommended growth and recombinant retrovirus production conditions see Cosset F et al, Journal of Virology, 1995, v69 pp7430-7436 & Takeuchi Y et al, Journal of Virology, 1994, v68 pp8001-8007
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Production of retroviral vectors for gene therapy with the human packaging cell line FLYRD18.
  • Cosset et al. 1995. J Virol. 69(12):7430-6. PMID: 7494248.
  • High-titer packaging cells producing recombinant retroviruses resistant to human serum.
  • Takeuchi et al. 1994. J Virol. 68(12):8001-7. PMID: 7966590.
  • Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell.