CMT 167 Cell Line
The CMT 167 Cell Line is a highly metastatic murine alveogenic lung carcinoma cell line, derived from cell line CMT64, used to study the origins and causes of metastasis.Background and…
Contributors
| Inventor | Institute |
|---|---|
| Peter Riddle | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| Cat. #: | 151448 |
|---|---|
| Tool sub type: | Primary |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Lung cancer;Lung cancer |
| Organism: | Mouse |
| Tissue: | Lung |
| Gender: | Female |
| Model: | Cancer cell line |
| Morphology: | Epithelial cell line, with closely packed sheets at confluence. |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Lung carcinoma;Lung carcinoma |
| Primary citation: | Layton and Franks. 1984. Br J Cancer. 49(4):415-21. PMID: 6324836. |
| Alternate name: | CMT167; CMT-167 |
|---|---|
| Product description: | CMT 167 is a highly metastatic murine alveogenic lung carcinoma cell line, derived from the parental CMT 64 line. Developed in 1984 at the Cancer Research UK London Research Institute (Lincoln's Inn Fields), CMT 167 was established through subcloning and in vivo selection for enhanced metastatic potential from pooled lung metastases of CMT 64. Compared to CMT 64, CMT 167 exhibits a markedly higher propensity for metastasis and is a useful tool to investigate the mechanisms and origins of metastatic progression in lung cancer. |
| Gender: | Female |
| CRISPR: | Yes |
| Conditional: | Yes |
| Production details: | CMT 167 is a subclone of the CMT 64 alveogenic lung carcinoma cell line, originally derived from a primary tumour in a C57BL/1CRF mouse. To generate CMT 167, adult female C57B/T mice (aged 4–6 months) were subcutaneously injected in the lower right flank using a Bashford needle. The injected material consisted of pooled tumour fragments taken from various non-necrotic regions of CMT 64-derived tumours. Following tumour development, cells were harvested from resulting lung metastases. Epithelial outgrowths from these metastatic sites were cultured and selectively expanded based on their high metastatic potential. Through this process of subcloning and in vivo screening, the CMT 167 cell line was established as a more aggressively metastatic variant of CMT 64. |
| Cellosaurus ID: | CVCL_2405 |
| disease: | Cancer;Cancer |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM with 2mM Glutamine and 10% FCS |
| Subculture routine: | Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10^4 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density 1.2 x 10^5 cells/cm². Please also see detailed protocol within the Product Datasheet in the Documentation section below. |
| Mycoplasma free: | Yes |
| References: |
Bullock et al. 2019. Life Sci Alliance. 27:2(3): e201900328. PMID: 31133614 Tippimanchai et al. 2018. Oncoimmunology. 7(6):e1438105. PMID: 29872579 Seshadri et al. 2018. Front Pharmacol. 9:759. PMID: 30061830 Li et al. 2017. Cancer Immunol Res. 9: 767-777. PMID: 28819064 Pelaz et al. 2017. ACS Nano. 11(3):2313-2381. PMID: 28290206 Evans et al. 2009. Cancer Res. 69(5):1733-8. PMID: 19208832 Ismail et al. 2000. Cancer Res. 60(5):1173-6. PMID: 10728668 Franks and Layton. 1984. Br J Cancer. 49(4):423-9. PMID: 6324837 Layton and Franks. 1984. Br J Cancer. 49(4):415-21. PMID: 6324836 Franks et al. 1976. Cancer Res. 36(3):1049-55. PMID: 1253168 |
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Product Images
CMT 167 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)
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