MEF RAF1 KO Cell Line
Exclusive ToolThe MEF RAF1 KO Cell Line allows in vivo study of Raf-1 knockout and Ras signalling and can be used to carry out drug screening toxicology studies.
Contributors
| Inventor | Institute |
|---|---|
| Catrin Pritchard | University of Leicester |
| SKU: | 151576 |
|---|---|
| Product description: | The MEF RAF1 KO Cell Line allows in vivo study of Raf-1 knockout and Ras signalling and can be used to carry out drug screening toxicology studies. MEF are produced from the Raf-1+/- knockout mouse. |
| Alternate name: | Raf-1-/- MEF; MEF RAF1 KO |
| Conditional: | Yes |
| Production details: | Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4ÄÂ?°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture. |
| Cellosaurus ID: | CVCL_6D89 |
| Disease: | Cancer |
| Cat. #: | 151576 |
|---|---|
| Provenance and Ownership: | The MEF RAF1 KO Cell Line is owned by Cancer Research UK. CancerTools supplies the MEF RAF1 KO Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing MEF RAF1 KO Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Cancer;Cell signaling and signal transduction;Drug development;Metabolism |
| Organism: | Mouse |
| Tissue: | Embryo |
| Model: | Knock-Out |
| Format: | Frozen |
|---|---|
| Storage conditions: | Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability. |
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM, 10% FCS, 100U/mol penicillin/streptomycin |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227. MEK kinase activity is not necessary for Raf-1 function. |
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MEF RAF1 KO Cell Line. Early log. phase.
MEF RAF1 KO Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
