#151576

MEF RAF1 KO Cell Line

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Cat. #151576

MEF RAF1 KO Cell Line

Cat. #: 151576

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Mouse

Tissue: Embryo

Disease: Cancer

Model: Knock-Out

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Catrin Pritchard

Institute: University of Leicester

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MEF RAF1 KO Cell Line
  • Research fields: Cancer;Cell signaling and signal transduction;Drug development;Metabolism
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer
  • Model: Knock-Out
  • Conditional: Yes
  • Description: The MEF RAF1 KO Cell Line allows in vivo study of Raf-1 knockout and Ras signalling and can be used to carry out drug screening toxicology studies. MEF are produced from the Raf-1+/- knockout mouse.
  • Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4Â?‚°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
  • Biosafety level: 1
  • Cellosaurus id: CVCL_6D89

Target Details

  • Target: RAF1

Applications

  • Application notes: See the Raf-1 knockout mouse in related products.

Handling

  • Format: Frozen
  • Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
  • Mycoplasma free: Yes

Related Tools

  • Related tools: Raf-1 KO Mouse

References

  • Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
  • MEK kinase activity is not necessary for Raf-1 function.