MEF RAF1 KO Cell Line

The MEF RAF1 KO Cell Line allows in vivo study of Raf-1 knockout and Ras signalling and can be used to carry out drug screening toxicology studies.

Contributors

Inventor Institute
Catrin Pritchard University of Leicester
Cat. #: 151576
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Cell signaling and signal transduction;Drug development;Metabolism
Organism: Mouse
Tissue: Embryo
Model: Knock-Out
Alternate name: Raf-1-/- MEF; MEF RAF1 KO
Product description: The MEF RAF1 KO Cell Line allows in vivo study of Raf-1 knockout and Ras signalling and can be used to carry out drug screening toxicology studies. MEF are produced from the Raf-1+/- knockout mouse.
Conditional: Yes
Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4Â?‚°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
Cellosaurus ID: CVCL_6D89
Disease: Cancer
Format: Frozen
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry ice
Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
Mycoplasma free: Yes
Biosafety level: 1
References:

Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.

MEK kinase activity is not necessary for Raf-1 function.

Images

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MEF RAF1 KO Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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