H357 Cell Line

H357 cell line is highly responsive to TGF beta and undergoes epithelial to mesenchymal transition. It expresses high levels of TGF beta1.

Contributors

Inventor Institute
Stephen Prime University of Bristol
Cat. #: 153422
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Cancer types: Head and neck cancer
Research Fields: Cancer;Cancer
Organism: Canine
Tissue: Tongue
Gender: Male
Model: Tumourigenic cell line
Growth properties: Adherent
Alternate name: H-357; h357
Product description: H357 was established from a squamous cell carcinoma (SCC) of the tongue (20mm) of a 74 year-old male patient. H357 has the following features: polygonal morphology, STNMP stage I, well differentiated and node negative tumour. This cell line has mutant p53, codon 110 exon 4, G to A; the previously reported mutant Ha-ras status of this cell line: codon: 13, G to A, codon 61, A-G is under investigation. H357 is highly responsive to TGF beta and undergoes epithelial to mesenchymal transition. H357 cells are tumourigenic in athymic nude mice on subcutaneous injection and non-tumourigenic on orthotopic injection. Haplotype information: A*02,A*31; B*40,B*44; Cw*03,Cw*05. Explore more human oral SCC cell lines from the same inventor including: H157, H376, H400, H314 and H103.
Gender: Male
CRISPR: Yes
Conditional: Yes
Cellosaurus ID: CVCL_2462
Disease: Cancer;Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate.
Subculture routine: Split sub-confluent cultures (70-80%), approximately every 5-6 days, 1:8 to 1:10 using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Suggested seeding density of 5 x 1000 cells/cm². Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability.
Mycoplasma free: Yes
References:

Fahey et al. 1996. Br J Cancer. 74(7):1074-80. PMID: 8855977.

Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31(2):136-143. PMID: 7633286.

Prime et al. 1994. Int J Cancer. 56(3):406-412. PMID: 7508893.

Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.

Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-67. PMID: 8180579.

Prime et al. 1990. J Pathol. 160(3):259-269. PMID: 1692339.

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