H357 Cell Line

Cat. #153422

H357 Cell Line

Cat. #: 153422

Unit size: 1x10^6 cells / vial

Tissue: Tongue

Disease: Cancer

Model: Tumour line


This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.


Inventor: Stephen Prime

Institute: University of Bristol

Tool Details
Target Details

Tool Details


  • Name: H357 Cell Line
  • Research fields: Cancer
  • Tissue: Tongue
  • Disease: Cancer
  • Growth properties: Adherent
  • Model: Tumour line
  • Conditional: Yes
  • Description: Established from a squamous cell carcinoma (SCC) of the tongue (20mm) of a 74 year-old male patient. STNMP stage I, well differentiated, node negative tumour. Mutant p53, codon 110 exon 4, G to A; the previously reported mutant Ha-ras status of this cell line: codon: 13, G to A, codon 61, A-G is under investigation. This cell line is highly responsive to TGF beta and undergoes epithelial to mesenchymal transition. It expresses high levels of TGF beta1. Fahey et al (1996). Tumourigenic in athymic nude mice on subcutaneous injection and non-tumorigenic on orthotopic injection. Haplotype information: A*02,A*31; B*40,B*44; Cw*03,Cw*05
  • Cellosaurus id: CVCL_2462

Target Details

  • Target: Human oral squamous cell carcinoma, tongue


  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%), approximately every 5-6 days, 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37?‚° C. Suggested seeding density 5 x 1000 cells/cm?‹Â›. Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum ...
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice


  • Fahey et al. 1996. Br J Cancer. 74(7):1074-80. PMID: 8855977.
  • Dysregulation of autocrine TGF-beta isoform production and ligand responses in human tumour-derived and Ha-ras-transfected keratinocytes and fibroblasts.
  • Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
  • Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
  • Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893.
  • TGF-beta receptor regu...