Omega-E Packaging Cell Line

The Omega-E Packaging Cell Line ias an helper-free packaging cell line & shuttle vector system enabling high-titer production of recombinant retroviral vectors for mammalian gene transfer, with significantly reduced probability of replication-competent retrovirus generation.

Contributors

Inventor Institute
Hartmut Land, Jay Morgenstern Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151463
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Genetics
Organism: Mouse
Tissue: Embryo
Model: Packaging
Growth properties: Recombinant retroviral production
Product description: Helper-free packaging cell line developed by Morgenstern and Land in 1990 for safe, high-titre pBABE vector production. Features split gag-pol/env genes that minimise replication competent virus production, while producing consistent viral titers of 10^6-10^7 cfu/ml. Key component of the proven pBABE retroviral system for custom gene expression experiments. Use with any of the pBABE vectors (puro, neo, hygro and zeo) for complete retroviral gene delivery capabilities.
Conditional: Yes
Production details: The Omega E and pBabe recombinant retroviral system utilises shuttle vectors with multiple drug selection markers (the pBabe vector series) for increased flexibility of gene transfer vector design, and a third generation, helper-free packaging cell line (Omega E) derived from mouse NIH 3T3 cell lines, enabling high-titer production of Moloney murine leukemia virus for use in mammalian gene transfer
Cellosaurus ID: CVCL_2151
Parental cell line: NIH/3T3
Format: Frozen
Shipping conditions: Dry ice
Growth medium: For recommended growth and recombinant retrovirus production conditions, see Morgenstern & Land. 1990. Nucleic Acids Research. 18:3587-96. PMID: 2194165
References:

Littlewood et al. 1995. Nucleic Acids Res. 23(10):1686-90. PMID: 7784172.

Morgenstern et al. 1990. Nucleic Acids Res. 18(12):3587-96. PMID: 2194165.

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