Gliosarcoma WHO IV cell line, NCH57

Gliosarcoma WHO IV cell line, NCH57

Contributors

Institute
Heidelberg University
Cat. #: 162334
Cancer types: Brain
Organism: Human
Tissue: Brain
Morphology: Adherent
Growth properties: Cells grow as adherent monolayer cultures under standard culture conditions at 37°C in a humidified incubator with 5% CO?. Medium is replaced twice per week.
Primary citation: Schule et al. 2012. Scientific World Journal. 2012:697313. PMID: 22919349.
Product description: Gliosarcoma WHO IV cell line, NCH57
Production details: Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro.
Growth medium: DMEM (high glucose) supplemented with 10% fetal calf serum (FCS), 1% GlutaMAX, and 1% penicillin/streptomycin.
Subculture routine: Cells are passaged at sub-confluent densities using 0.05% Trypsin-EDTA. After enzymatic detachment, trypsin is neutralised with serum-containing medium and cells are reseeded into fresh culture vessels. Passage number should be recorded.
Temperature: 37°C
References:

Schule et al. 2012. Scientific World Journal. 2012:697313. PMID: 22919349.

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