PEO1 Cell Line

PEO1 is an adherent cell line derived from a malignant effusion from the peritoneal ascites of a patient with high-grade serous ovarian carcinoma (historically described as serous adenocarcinoma).

Contributors

Inventor Institute
Simon Langdon Cancer Research UK Edinburgh Centre
Cat. #: 151672
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Organism: Human
Tissue: Ovary
Gender: Male
Model: Tumourigenic
Morphology: Epithelial; islands of uniform polygonal cells
Growth properties: Adherent
Primary citation: Langdon et al. 1988. Cancer Res. 48(21):6166-72. PMID: 3167863
Product description: The PEO1 Cell Line is one of nine in the PE ovarian cancer panel (derived from 4 patients at varying stages of disease, isolated from different malignant sites and treatment timepoints), providing a model system for the study of ovarian cancer biology and therapeutic response. PEO1 is an adherent cell line derived from a malignant effusion from the peritoneal ascites of a patient with high-grade serous ovarian carcinoma (historically described as serous adenocarcinoma). The patient previously received cisplatin, 5-fluorouracil and chlorambucil treatment. PEO1 is tumourigenic in immunologically-deprived CBA mice and represents a platinum-sensitive baseline model within the PEO cell line series used to study treatment-driven tumour evolution. This is a slow growing cell line, please refer to the sub-culturing notes below.
Gender: Male
CRISPR: Yes
Conditional: No
Additional notes: Please be aware that the originating laboratory of the PEO1 cell line acknowledges that PEO1 is both genetically unstable and derived from a heterogeneous population that was already present in the patient at the time of biopsy. This is evident in the literature (Cooke et al., 2010). Genetic differences within the PEO1, PEO4 and PEO6 cell lines suggest that PEO4 and PEO6 are not direct descendants of PEO1 but have diverged from a common ancestor.
Cellosaurus ID: CVCL_2686
Model description: The cell line is tumourigenic in immunologically-deprived CBA mice
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: RPMI-1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Foetal Bovine Serum (FBS)
Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 10^4 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time approximately 37 hours
Temperature: 37°C
Mycoplasma free: Yes
Biosafety level: 1
References:

Cruz et al. 2017. Cancer Genomics Proteomics. 14(1):35-51. PMID: 28031236

Coscia et al. 2016. Nat Commun. 7:12645. PMID: 27561551

Matassa et al. 2016. Cell Death Differ. :. PMID: 27206315

Szikriszt et al. 2016. Genome Biol. 17(1):99. PMID: 27161042

Huang et al. 2015. Oncotarget. :. PMID: 26683361

Nevedomskaya et al. 2015. J Proteome Res. :. PMID: 26629888

Patel et al. 2015. Cell Oncol (Dordr): PMID: 26266765

Hearn et al. 2015. Proc Natl Acad Sci U S A. :. PMID: 26162681

Ren et al. 2015. J Steroid Biochem Mol Biol. 150:54-63. PMID: 25817828

Huntoon et al. 2013. Cancer Res. 73(12):3683-91. PMID: 23548269

Cooke SL et al. 2010 Oncogene 2,29(35):4905-13. PMID: 20581869

Sakai W et al. 2009. Cancer Res.15, 69(16): 6381–6386. PMID: 19654294

Langdon et al. 1990. Br J Cancer. 62(2):213-6. PMID: 2386737

Langdon et al. 1988. Cancer Res. 48(21):6166-72. PMID: 3167863

Images

View Gallery
PEO1 Cell Line. 3 days post plating. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

Related Tools for Cell lines

Cat. # Tool Name
151014 MCF7 AREc32 Cell Line Key Info View Tool
151446 FLYRD18 Cell Line Key Info View Tool
151503 1-7 CE1 Cell Line Key Info View Tool
151558 MEF Trex1 KO Cell Line Key Info View Tool
151448 CMT 167 Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.