PEO1 Cell Line

A BRCA2-mutant, HR-deficient serous ovarian cancer model used as a therapy-sensitive comparator for DNA repair, platinum sensitivity and PARP inhibitor sensitivity studies. Only available from CancerTools. Save 30% on this cell line this summer. Use code SUM26CELL at checkout.

Contributors

Inventor Institute
Simon Langdon Cancer Research UK Edinburgh Centre
SKU: 151672
Product description: PEO1 is a patient-derived high-grade serous ovarian cancer cell line established from malignant ascites. It carries a BRCA2 mutation and is homologous recombination-deficient, making it a useful therapy-sensitive comparator for studies of DNA repair, platinum sensitivity and PARP inhibitor response. As part of the patient-matched PEO1/PEO4/PEO6 series, PEO1 supports comparative studies of treatment response, acquired resistance and ovarian cancer evolution.
Gender: Female
Conditional: No
Additional notes: Please be aware that the originating laboratory of the PEO1 cell line acknowledges that PEO1 is both genetically unstable and derived from a heterogeneous population that was already present in the patient at the time of biopsy. This is evident in the literature (Cooke et al., 2010). Genetic differences within the PEO1, PEO4 and PEO6 cell lines suggest that PEO4 and PEO6 are not direct descendants of PEO1 but have diverged from a common ancestor. PEO1 has also been reported to be tumourigenic in immunologically deprived CBA mice and is a slow-growing cell line; please refer to the sub-culturing notes for recommended handling guidance.
Cellosaurus ID: CVCL_2686
Model description: The cell line is tumourigenic in immunologically-deprived CBA mice
Disease: Cancer
Cat. #: 151672
Provenance and Ownership: The PEO1 Cell Line is owned by Cancer Research UK. CancerTools supplies the PEO1 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing PEO1 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Cancer types: Ovarian cancer
Organism: Human
Tissue: Ovary
Gender: Female
Model: Tumourigenic
Morphology: Epithelial; islands of uniform polygonal cells
Growth properties: Adherent
Cancer Types In Detail: High-Grade Serous Ovarian (HGSOC)
Primary citation: Langdon et al. 1988. Cancer Res. 48(21):6166-72. PMID: 3167863
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: RPMI-1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Foetal Bovine Serum (FBS)
Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 10^4 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time approximately 37 hours
Temperature: 37°C
Mycoplasma free: Yes
Biosafety level: 1
References:

Cruz et al. 2017. Cancer Genomics Proteomics. 14(1):35-51. PMID: 28031236

Coscia et al. 2016. Nat Commun. 7:12645. PMID: 27561551

Matassa et al. 2016. Cell Death Differ. PMID: 27206315

Szikriszt et al. 2016. Genome Biol. 17(1):99. PMID: 27161042

Huang et al. 2015. Oncotarget. :. PMID: 26683361

Nevedomskaya et al. 2015. J Proteome Res.

PMID: 26629888

Patel et al. 2015. Cell Oncol (Dordr): PMID: 26266765

Hearn et al. 2015. Proc Natl Acad Sci U S A. PMID: 26162681

Ren et al. 2015. J Steroid Biochem Mol Biol. 150:54-63. PMID: 25817828

Huntoon et al. 2013. Cancer Res. 73(12):3683-91. PMID: 23548269

Cooke SL et al. 2010 Oncogene 2,29(35):4905-13. PMID: 20581869

Sakai W et al. 2009. Cancer Res.15, 69(16): 6381–6386. PMID: 19654294

Langdon et al. 1990. Br J Cancer. 62(2):213-6. PMID: 2386737

Langdon et al. 1988. Cancer Res. 48(21):6166-72. PMID: 3167863

Images

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PEO1 Cell Line. 3 days post plating. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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