H400 Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: H400 Cell Line
• Alternate name: h400; H-400
• Cancer: Head and neck cancer
• Research fields: Cancer
• Organism: Human
• Gender: Female
• Tissue: The alveolar process of the maxilla
• Donor: Female, 55 y.o. Clinical features: site: alveolar process; size (mm): 20-40; nodes: - ; metastases: - ; pathology: moderately differentiated; grade: II
• Disease: Cancer
• Growth properties: Adherent
• Model: Tumour cell line
• Model description: Mutant p53, codon 283 exon 8, C to G wild type K-, N- and Ha-ras. Non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth.
• Crispr: No
• Conditional: No
• Description: Established from a squamous cell carcinoma (SCC) of the alveolar process (20mm - 40mm) of a female patient aged 55. STNMP stage II, moderately differentiated, node negative tumour. This cell line is highly responsive to TGF-beta. These cells are non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth
• Application: Disease modeling, malignant progression studies, gene mutation and expression analysis
• Additional notes: Haplotype information: A*11,A*29; B*07,B*15; Cw*03,Cw*15
• Cellosaurus id: CVCL_2464

Target Details

• Target: Human oral squamous cell carcinoma, alveolar process

Applications

• Application: Disease modeling, malignant progression studies, gene mutation and expression analysis

Handling

• Format: Frozen
• Volume: 1 ml
• Growth medium: DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
• Temperature: 37° C
• Atmosphere: 5% CO2
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Subculture routine: Split sub-confluent cultures (70-80%) 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37C. Suggested seeding density 5 x 1000 cells/cm2. Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability
• Mycoplasma free: Yes

References

• Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286
• Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893
• Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215
• Yeudall et.al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-7. PMID: 8180579
• Prime et.al. 1990. J Pathol. 160(3):259-69. PMID: 1692339