U-2 OS Gal4-p300 Cell Line

U-2 OS Gal4-p300 Cell Line is a U2-OS cell expressing Gal4-p300. Available from the non-profit biorepository CancerTools.org

Contributors

Inventor Institute
Yili Yin University of Dundee
Cat. #: 151579
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Sarcoma
Research Fields: Genetics
Organism: Human
Model: Transgenic
Cancer Types In Detail: Osteosarcoma
Product description: U2-OS cells expressing Gal4-p300. p300 is a transcriptional coactivator that functions as integrator of numerous signaling pathways and are utilized by many DNA binding proteins to facilitate transcriptional activation. p300 shares numerous conserved domains with CREB binding protein (CBP), which is also a transcriptional coactivator. These shared domains include a histone acetyl transferase (HAT) domain, a bromo domain, and three cysteine- and histidine-rich domains. CBP also interacts with the RNA polymerase II holoenzyme and p300/CBP both contain transcriptional activation domains that function independently of HAT activity.
Production details: U2-OS cells were co-transfected with both the Gal4p300CRD1 expression vector (zeocin selection marker, backbone: pcDNA4/TO) and the Gal4-EIB-luciferase reporter vector (neomycin selection marker, backbone: pCG4 without the CMV promoter) using Fugene 6 transfection reagent.24 hours after transfection, stable transfectants were selected using G418 3.0 mg /ml and Zeocin 3.0 mg/ml until massive cell death, and then grown under G418 0.5 mg /ml and Zeocin 0.5 mg/ml for further selection. Establishe…
Parental cell line: U-2 OS
disease: Cancer
Format: Frozen
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry ice
Growth medium: DMEM plus 10% Foetal Bovine Serum and 1% Penicillin-Streptomycin
Recommended controls: U-2 OS parental line
References:

Girdwood et al. 2003. Mol Cell. 11(4):1043-54. PMID: 12718889.

P300 transcriptional repression is mediated by SUMO modification.

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