#151665

MEF PKCe KO Cell Line

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Cat. #151665

MEF PKCe KO Cell Line

Cat. #: 151665

Unit size: 1x10^6 cells / vial

Organism: Mouse

Tissue: Embryo

Disease: Cancer

Model: Knock-Out

£575.00

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Contributor

Inventor: Peter Parker

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MEF PKCe KO Cell Line
  • Research fields: Cancer;Cell biology;Cell signaling and signal transduction
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer
  • Model: Knock-Out
  • Description: The MEF PKCe KO Cell Line is useful for studying the role of PKCe in various biological processes. Clonal MEFs have been derived from PKCepsilon knock-out embryos and subsequently stably transfected with empty pcDNA3 CMV/IE hygo+. Matched isogenic cell line with PKCe re-expressed is also available. Cells have reduced migratory capacity compared to their PKCe-expressing counterparts. Cells were used to demonstrate a role for PKCe in controlling the traffic of beta1 integrins in motile cells
  • Production details: MEFs were derived from PKCe knockout embryos. Polyclonal PKCeKO cells were transfected with pcDNA3 CMV/IE hygro+ vector using calcium phosphate. Clonal stable cell lines were selected using limiting dilution.
  • Cellosaurus id: CVCL_AS81

Target Details

  • Target: PKC epsilon

Handling

  • Format: Frozen
  • Growth medium: Cells were selected, and are routinely cultured in DMEM + 10% FCS and 100ug/ml hygromycin at 30degC in a 10% CO2 atmosphere
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Cited by 24 subsequent publications including:
  • Ivaska, J. et al. 2002. EMBO J. 21:3608-19.PMID: 12110574
  • Kermorgant, S. et al. 2004. EMBO J. 23: 3721:34. PMID 15385963
  • Pardo et al. 2006. EMBO J. 25(13):3078-88. PMID: 16810323.
  • FGF-2 protects small cell lung cancer cells from apoptosis through a complex involving PKCepsilon, B-Raf and S6K2.