MEF PKCe KO Cell Line

The MEF PKCe KO Cell Line is useful for studying the role of PKCe in various biological processes. Clonal MEFs have been derived from PKCepsilon knock-out embryos and subsequently stably transfected with empty pcDNA3 CMV/IE hygo+.

Contributors

Inventor Institute
Peter Parker Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151665
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Cell biology;Cell signaling and signal transduction
Organism: Mouse
Tissue: Embryo
Model: Knock-Out
Product description: The MEF PKCe KO Cell Line is useful for studying the role of PKCe in various biological processes. Clonal MEFs have been derived from PKCepsilon knock-out embryos and subsequently stably transfected with empty pcDNA3 CMV/IE hygo+. Matched isogenic cell line with PKCe re-expressed is also available. Cells have reduced migratory capacity compared to their PKCe-expressing counterparts. Cells were used to demonstrate a role for PKCe in controlling the traffic of beta1 integrins in motile cells
Production details: MEFs were derived from PKCe knockout embryos. Polyclonal PKCeKO cells were transfected with pcDNA3 CMV/IE hygro+ vector using calcium phosphate. Clonal stable cell lines were selected using limiting dilution.
Cellosaurus ID: CVCL_AS81
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Cells were selected, and are routinely cultured in DMEM + 10% FCS and 100ug/ml hygromycin at 30degC in a 10% CO2 atmosphere
References:

Cited by 24 subsequent publications including:

Ivaska, J. et al. 2002. EMBO J. 21:3608-19.PMID: 12110574

Kermorgant, S. et al. 2004. EMBO J. 23: 3721:34. PMID 15385963

Pardo et al. 2006. EMBO J. 25(13):3078-88. PMID: 16810323.

FGF-2 protects small cell lung cancer cells from apoptosis through a complex involving PKCepsilon, B-Raf and S6K2.

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