Cat. #153419
H103 Cell Line
Cat. #: 153419
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Tissue: Tongue
Disease: Cancer
Model: Tumourigenic cell line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Stephen Prime
Institute: University of Bristol
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: H103 Cell Line
- Alternate name: h103; H-103
- Research fields: Cancer
- Tissue: Tongue
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumourigenic cell line
- Description: Established from a small squamous cell carcinoma (SSC) of the tongue (<20mm in size) of a 32 year-old male patient. STNMP stage 1, well differentiated, node negative tumour. Aneuploid- dual G-/G1 peak on cell cycle analysis by flow cytometry. Mutant p53, codon 244 exon 7; G to T; wild type K-, N- and Ha-ras. Tumourigenic in athymic nude mice, both by subcutaneous injection and injection into the floor of the mouth. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
- Cellosaurus id: CVCL_2457
Target Details
- Target: Human oral squamous cell carcinoma, tongue
Handling
- Format: Frozen
- Growth medium: Split sub-confluent cultures (70-80%) 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37?°C. Suggested seeding density 5 x 1000 cells/cm? . Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydroco...
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-143. PMID: 7633286.
- Prime et al. 1994. Int J Cancer. 56(3):406-412. PMID: 7508893.
- Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.
- Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-67. PMID: 8180579.
- Prime et al. 1990. J Pathol. 160(3):259-269. PMID: 1692339.
