Cat. #151480
HEK293A EGFP-LC3 Cell Line
Cat. #: 151480
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Embryo
Disease: Cancer
Model: Transgenic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Sharon Tooze
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: HEK293A EGFP-LC3 Cell Line
- Research fields: Apoptosis and autophagy;Cancer;Cell biology;Cell signaling and signal transduction;Drug development;Metabolism
- Tool sub type: Continuous
- Parental cell: HEK293
- Organism: Human
- Tissue: Embryo
- Disease: Cancer
- Growth properties: Autophagy
- Model: Transgenic
- Conditional: Yes
- Description: HEK 293A EGFP-LC3 is a stable epithelial cell line expressing EGFP-tagged LC3 (rat) in a HEK293A background. This line functions as a reporter cell line to monitor the induction of autophagy after aminoacid starvation or rapamycin treatment as well as autophagosome formation. This cell line uses GFP-LC3 as a standard assay read out and can be evaluated by both immunofluorescence and biochemical methods.
- Production details: EGFP-LC3 (rat) plasmid was transfected into HEK293A cells. The cells were selected with 800ug/ml geneticin. Single cell clones were selected and grown up. Several clones were tested and clone 2GL9 was taken as the best based on a number of parameters (inc. GFP-LC3 expression levels and responsiveness to amino acid deprivation).
- Additional notes: HEK293 cells were obtained under licence from AdVec
- Recommended controls: HEK293-A parental line
- Cellosaurus id: CVCL_W347
Target Details
- Target: GFP-LC3B (Rat)
Applications
- Application notes: Stable cell line expressing EGFP-tagged LC3 (rat) in background of HEK293A cell line.
Handling
- Format: Frozen
- Growth medium: DMEM + 10% FCS + 2mM Glutamine. (400ug/ml geneticin can be added for maintainance but this is not normally necessary as the GFP-LC3 expression level is maintained over at least 20 passages).
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Chan et al. 2007. J Biol Chem. 282(35):25464-74. PMID: 17595159.
- siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy.
- Kchl et al. 2006. Traffic. 7(2):129-45. PMID: 16420522.
- Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomes.
