HEK293A EGFP-LC3 Cell Line

HEK 293A EGFP-LC3 is a stable epithelial cell line expressing EGFP-tagged LC3 (rat) in a HEK293A background.

Contributors

Inventor Institute
Sharon Tooze Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151480
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Organism: Human
Tissue: Embryo
Gender: Male
Model: Transgenic
Growth properties: Autophagy
Product description: HEK 293A EGFP-LC3 is a stable epithelial cell line expressing EGFP-tagged LC3 (rat) in a HEK293A background. This line functions as a reporter cell line to monitor the induction of autophagy after aminoacid starvation or rapamycin treatment as well as autophagosome formation. This cell line uses GFP-LC3 as a standard assay read out and can be evaluated by both immunofluorescence and biochemical methods.
Gender: Male
CRISPR: Yes
Conditional: Yes
Production details: EGFP-LC3 (rat) plasmid was transfected into HEK293A cells. The cells were selected with 800ug/ml geneticin. Single cell clones were selected and grown up. Several clones were tested and clone 2GL9 was taken as the best based on a number of parameters (inc. GFP-LC3 expression levels and responsiveness to amino acid deprivation).
Additional notes: HEK293 cells were obtained under licence from AdVec
Cellosaurus ID: CVCL_W347
Parental cell line: HEK293
disease: Cancer;Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 10% FCS + 2mM Glutamine. (400ug/ml geneticin can be added for maintainance but this is not normally necessary as the GFP-LC3 expression level is maintained over at least 20 passages).
Recommended controls: HEK293-A parental line
Mycoplasma free: Yes
References:

Chan et al. 2007. J Biol Chem. 282(35):25464-74. PMID: 17595159.

siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy.

Kchl et al. 2006. Traffic. 7(2):129-45. PMID: 16420522.

Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomes.

Images

View Gallery
HEK293A EGFP-LC3 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

Related Tools for Cell lines

Cat. # Tool Name
151014 MCF7 AREc32 Cell Line Key Info View Tool
151447 CMT 64 Cell Line Key Info View Tool
151448 CMT 167 Cell Line Key Info View Tool
151450 JIM3 Cell Line Key Info View Tool
151463 Omega-E Packaging Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.