MM032 cell line

Unpigmented cell line derived from a skin metastasis with superficial spreading melanoma (SSM) with intrinsic Resistance to BRAFi. This cell line displays a neural crest like phenotype.

Contributors

Inventor Institute
Ghanem Elias, Ahmad Najem Université libre de Bruxelles
Cat. #: 162369
Cancer types: Melanoma
Organism: Human
Tissue: Skin
Gender: Female
Model: Tumour cell line
Growth properties: Adherent
Cancer Types In Detail: Melanoma
Primary citation: Wouters, J et al. Nat Cell Biol 2020. 22, 986–998.PMID: 32753671.
Product description: Unpigmented cell line derived from a skin metastasis with superficial spreading melanoma (SSM) with intrinsic Resistance to BRAFi. This cell line displays a neural crest like phenotype.
Gender: Female
CRISPR: No
disease: Melanoma
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: Ham’s F10 supplemented with 10% of fetal calf serum (FCS), with L-glutamine (1mM), penicillin (100 IU/ml), and streptomycin (100 µg/ml). Renewal 2 to 3 times per week
Subculture routine: Split sub-confluent cultures (70-80%) 1:2 to 1:5
Temperature: 37°C
Atmosphere: 5% CO2
Biosafety level: 2
References:

Najem et al. 2023. Cells 12, 1855. PMID: 37111048

Zalfa et al. 2017. Cell Death Dis 8, e3169. PMID: 28912503

Sabbah et al. 2021. Mol Cancer Res 19, 1221–1233. PMID: 34021077

Rambow et al. 2018. Cell 174, 843–855.e19. PMID: 30033366

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