Cat. #160740
BRCA1 c.3612delA iPSC line cell line (2042)
Cat. #: 160740
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Fibroblast
Disease: Hereditary breast cancer
Model: Cancer Model
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Maria Carmo-Fonseca ; Sim?Âo Teixeira da Rocha
Institute: Instituto de Medicina Molecular Jo?Âo Lobo Antunes
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: BRCA1 c.3612delA iPSC line cell line (2042)
- Alternate name: BRCA1 c.3612delA iPSC line (242) ÄË?Â? clones 9, 1, 4 and 5 (IBBISTi1-A, IBBISTi1-B, IBBISTi1-C and IBBISTi1-D)
- Research fields: Cancer;Genetics;Stem cell biology
- Parental cell: Human fibroblasts
- Organism: Human
- Tissue: Fibroblast
- Disease: Hereditary breast cancer
- Model: Cancer Model
- Conditional: No
- Description: BRCA1-associated cancer model. Investigation of tissue-specific stem/progenitor cells that represent the cells of origin of BRCA1-associated tumours, modelling early phases of malignant transformation and development of drug screening strategies aiming at preventing BRCA1 mutant hereditary cancers.
- Production details: Reprogramming of human induced pluripotent stem cells from a skin biopsy of a female donor heterozygous for the BRCA1 mutation c.3612delA.
- Recommended controls: Yes, a familiar healthy control (2040).
Target Details
- Target: Hereditary breast and ovarian cancer
Handling
- Format: Frozen
- Growth medium: Cells are cultured on MatrigelTM (Corning)-coated plates with mTeSRTMPlus Medium (StemCell Technologies). Medium is changed daily. Cells are passaged every 3ÄË?Â?Â4 days (when the colonies covered approximately 85% of the surface area of the culture dish) using 0.5 mM EDTA dissociation buffer (Life Technologies). Cells are incubated at 37 ÄË?ÂÄÂC in a humidified 5% CO2 incubator.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
