#151559

MEF UNG KO Cell Line

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Cat. #151559

MEF UNG KO Cell Line

Cat. #: 151559

Unit size: 1x10^6 cells / vial

Organism: Mouse

Tissue: Embryo

Disease: Cancer

Model: Knock-Out

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Thomas Lindahl

Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MEF UNG KO Cell Line
  • Cancer: Blood cancer
  • Cancers detailed: B cell lymphoma
  • Research fields: Genetics
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer
  • Model: Knock-Out
  • Description: The MEF UNG KO Cell Line is a tool for in vivo study of the function of UNG and the effects of DNA mismatch during DNA synthesis. UNG encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway.
  • Production details: Primary MEFs were prepared from embryos, and permanent cell lines established from transformed clones arising spontaneously after repeated passage in culture, by standard procedures

Target Details

  • Target: Uracil-DNA Glycosylase (UNG)

Handling

  • Format: Frozen
  • Growth medium: DMEM:F12 (1:1) + 10% FCS.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.

References

  • Nilsen et al. 2003. Oncogene. 22(35):5381-6. PMID: 12934097.
  • Gene-targeted mice lacking the Ung uracil-DNA glycosylase develop B-cell lymphomas.