MEF UNG KO Cell Line

The MEF UNG KO Cell Line is a tool for in vivo study of the function of UNG and the effects of DNA mismatch during DNA synthesis. UNG encodes one of several uracil-DNA glycosylases.

Contributors

Inventor Institute
Thomas Lindahl Cancer Research UK, London Research Institute: Clare Hall Laboratories
Cat. #: 151559
Unit size: 1×10^6 cells / vial
Cancer types: Blood cancer
Research Fields: Genetics
Organism: Mouse
Tissue: Embryo
Model: Knock-Out
Cancer Types In Detail: B cell lymphoma
Product description: The MEF UNG KO Cell Line is a tool for in vivo study of the function of UNG and the effects of DNA mismatch during DNA synthesis. UNG encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway.
Production details: Primary MEFs were prepared from embryos, and permanent cell lines established from transformed clones arising spontaneously after repeated passage in culture, by standard procedures
disease: Cancer
Format: Frozen
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry ice
Growth medium: DMEM:F12 (1:1) + 10% FCS.
References:

Nilsen et al. 2003. Oncogene. 22(35):5381-6. PMID: 12934097.

Gene-targeted mice lacking the Ung uracil-DNA glycosylase develop B-cell lymphomas.

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