MM099 cell line

Unpigmented cell line derived from a skin metastasis. This cell line displays an invasive/Mesenchymal_like phenotype.

Contributors

Inventor Institute
Ghanem Elias, Ahmad Najem Université libre de Bruxelles
Cat. #: 162371
Cancer types: Melanoma
Organism: Human
Tissue: Skin
Gender: Female
Model: Tumour cell line
Growth properties: Adherent
Cancer Types In Detail: Melanoma
Primary citation: Wouters, J et al. Nat Cell Biol 2020. 22, 986–998.PMID: 32753671.
Product description: Unpigmented cell line derived from a skin metastasis. This cell line displays an invasive/Mesenchymal_like phenotype.
Gender: Female
CRISPR: No
disease: Melanoma
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: Ham’s F10 supplemented with 10% of fetal calf serum (FCS), with L-glutamine (1mM), penicillin (100 IU/ml), and streptomycin (100 µg/ml). Renewal 2 to 3 times per week
Subculture routine: Split sub-confluent cultures (70-80%) 1:2 to 1:5
Temperature: 37°C
Atmosphere: 5% CO2
Biosafety level: 2
References:

Wouters et al. 2020. Nat Cell Biol 22, 986–998. PMID: 32747822

Verfaillie et al. 2015. Nat Commun 6, 6683. PMID: 25865119

Zalfa et al. 2017. Cell Death Dis 8, e3169. PMID: 28912503

Marin-Bejar et al. 2021. Cancer Cell 39, 1135–1149.e8. PMID: 34111488

Pozniak et al. 2024. Cell 187, 166–183.e25. PMID: 38099355

Ennen et al. 2017. Clin Cancer Res 23, 7097–7107. PMID: 28974683

Purwin et al. 2023. iScience 26

107472. PMID: 37584125

Mauduit et al. 2021. eLife 10, e71735. PMID: 34932533

Berico et al. 2021. EMBO Rep 22, e51683. PMID: 33963251

Rambow et al. 2018. Cell 174, 843–855.e19. PMID: 30033366

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