Cat. #162371
MM099 cell line
Cat. #: 162371
Availability: 8-10 weeks
Organism: Human
Tissue: Skin
Disease: Melanoma
Model: Tumour cell line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Ghanem Elias, Ahmad Najem
Institute: Université libre de Bruxelles
Primary Citation: Wouters, J et al. Nat Cell Biol 2020. 22, 986–998.PMID: 32753671.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: MM099 cell line
- Cancer type: Skin cancer
- Cancers detailed: Melanoma
- Organism: Human
- Gender: Female
- Tissue: Skin
- Disease: Melanoma
- Growth properties: Adherent
- Model: Tumour cell line
- Crispr: No
- Description: Unpigmented cell line derived from a skin metastasis. This cell line displays an invasive/Mesenchymal_like phenotype.
- Application: High-throughput screening/Drug testing
- Biosafety level: 2
Applications
- Application: High-throughput screening/Drug testing
Handling
- Format: Frozen
- Growth medium: Ham’s F10 supplemented with 10% of fetal calf serum (FCS), with L-glutamine (1mM), penicillin (100 IU/ml), and streptomycin (100 µg/ml). Renewal 2 to 3 times per week
- Temperature: 37°C
- Atmosphere: 5% CO2
- Shipping conditions: Dry Ice
- Storage conditions: Liquid Nitrogen
- Subculture routine: Split sub-confluent cultures (70-80%) 1:2 to 1:5
- Cultured in antibiotics: Yes
- Str profiling: Cell Line Authentication was performed using STR profiling with AmpFLSTR™ Identifiler™ PCR Amplification Kit (Thermo Fisher Scientific). 16 independent PCR-systems (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, AMEL, D5S818, FGA, D19S433, vWA, TPOX, and D18S51) were investigated and analyzed (Eurofins Genomics, Germany). The STR profile can be provided.
References
- Wouters et al. 2020. Nat Cell Biol 22, 986–998. PMID: 32747822
- Verfaillie et al. 2015. Nat Commun 6, 6683. PMID: 25865119
- Zalfa et al. 2017. Cell Death Dis 8, e3169. PMID: 28912503
- Marin-Bejar et al. 2021. Cancer Cell 39, 1135–1149.e8. PMID: 34111488
- Pozniak et al. 2024. Cell 187, 166–183.e25. PMID: 38099355
- Ennen et al. 2017. Clin Cancer Res 23, 7097–7107. PMID: 28974683
- Purwin et al. 2023. iScience 26
- 107472. PMID: 37584125
- Mauduit et al. 2021. eLife 10, e71735. PMID: 34932533
- Berico et al. 2021. EMBO Rep 22, e51683. PMID: 33963251
- Rambow et al. 2018. Cell 174, 843–855.e19. PMID: 30033366
