Cat. #151450
JIM3 Cell Line
Cat. #: 151450
Sub-type: Primary
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Blood
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: M.S. Hamilton
Institute: University of Birmingham
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: JIM3 Cell Line
- Cancer: Blood cancer
- Cancers detailed: Human multiple myeloma
- Research fields: Cancer;Drug development;Genetics
- Tool sub type: Primary
- Organism: Human
- Gender: Female
- Tissue: Blood
- Disease: Cancer
- Model: Tumour line
- Conditional: Yes
- Description: JIM3 is a multiple myeloma cell line established from plasma myeloma cells derived from pleural fluid of an advanced multiple myeloma patient (Hamilton et al., 1991). JIM3 carries compound mutations in KRAS (G12D heterozygous) and TP53 (R273C homozygous) (Moreaux et al., 2011). The cell line exhibits deficient DNA mismatch repair capacity with reduced G/T repair activity, and microsatellite instability (Velangi et al 2004). JIM3 displays a plasma cell phenotype and is a model for studying compound genetic lesions, DNA repair deficiency and genomic instability in multiple myeloma research.
- Production details: Derived from plasma myeloma cells from pleural fluid of advanced multiple myeloma female patient.
- Cellosaurus id: CVCL_2533
Handling
- Format: Frozen
- Growth medium: Dexter culture medium, consisting of Fischer's medium + 20% FCS +10-7 M hydrocortisone sodium succinate. Seed at 2-9 x 10 5 cells/cm3 5% CO2; 37 °C
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Hamilton M.S. et al. 1991. Leukemia. 5:768-771. PMID: 1943229
- Bergsagel P.L. et al. 1996. Proc. Natl. Acad. Sci. U.S.A. 93:13931-13936. PMID: 8943038
- Sakai A. et al. 1998. Blood. 92:3410-3415. PMID: 9787181
- Kuipers J. et al. 1999. Cancer Genet. Cytogenet. 109:99-107. PMID: 10087940
- Drexler H.G. et al. 2000. Leuk. Res. 24:681-703. PMID: 10936422
- Chesi M. et al. 2001. Blood. 97:729-736. PMID: 11157491
- Velangi et al. 2004. Carcinogenesis. 25(10):1795-803. PMID: 15142887
- Bataille F.-R. et al. 2006. Haematologica. 91:1234-1240. PMID: 16956823
- Keats J.J. et al. 2007. Cancer Cell. 12:131-144. PMID: 17692805
- Dib A. et al. 2008. J. Natl. Cancer Inst. Monogr. 39:25-31. PMID: 18647998
- Leone et al. 2008. Clin Cancer Res. 14(19):6033-41. PMID: 18829482
- Moreaux J. et al. 2011. Haematologica. 96:574-582. PMID: 21173094
- Maiga S. et al. 2015. Cytometry A. 87:285-288. PMID: 25688540
- Tessoulin B. et al. 2018. J. Hematol. Oncol. 11:137.1-137.13. PMID: 30545397
- Sarin V. et al. 2020. Leukemia. 34:2754-2765. PMID: 32123307.