JIM3 Cell Line

JIM3 was established from plasma myeloma cells derived from pleural fluid of an advanced multiple myeloma patient, and is homoplastic with the cell line JIM1. It allows in vitro study and comparison of genetic instability in myeloma tumour lines and in vitro study of the effects of DNA repair deficiency in myeloma tumour lines.

Contributors

Inventor Institute
M.S. Hamilton University of Birmingham
Cat. #: 151450
Tool sub type: Primary
Unit size: 1×10^6 cells / vial
Cancer types: Blood cancer
Research Fields: Cancer;Drug development;Genetics
Organism: Human
Tissue: Blood
Gender: Female
Model: Tumour line
Cancer Types In Detail: Human multiple myeloma
Product description: JIM3 is a multiple myeloma cell line established from plasma myeloma cells derived from pleural fluid of an advanced multiple myeloma patient (Hamilton et al., 1991). JIM3 carries compound mutations in KRAS (G12D heterozygous) and TP53 (R273C homozygous) (Moreaux et al., 2011). The cell line exhibits deficient DNA mismatch repair capacity with reduced G/T repair activity, and microsatellite instability (Velangi et al 2004). JIM3 displays a plasma cell phenotype and is a model for studying compound genetic lesions, DNA repair deficiency and genomic instability in multiple myeloma research.
Gender: Female
Conditional: Yes
Production details: Derived from plasma myeloma cells from pleural fluid of advanced multiple myeloma female patient.
Cellosaurus ID: CVCL_2533
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Dexter culture medium, consisting of Fischer's medium + 20% FCS +10-7 M hydrocortisone sodium succinate. Seed at 2-9 x 10 5 cells/cm3 5% CO2; 37 °C
References:

Hamilton M.S. et al. 1991. Leukemia. 5:768-771. PMID: 1943229

Bergsagel P.L. et al. 1996. Proc. Natl. Acad. Sci. U.S.A. 93:13931-13936. PMID: 8943038

Sakai A. et al. 1998. Blood. 92:3410-3415. PMID: 9787181

Kuipers J. et al. 1999. Cancer Genet. Cytogenet. 109:99-107. PMID: 10087940

Drexler H.G. et al. 2000. Leuk. Res. 24:681-703. PMID: 10936422

Chesi M. et al. 2001. Blood. 97:729-736. PMID: 11157491

Velangi et al. 2004. Carcinogenesis. 25(10):1795-803. PMID: 15142887

Bataille F.-R. et al. 2006. Haematologica. 91:1234-1240. PMID: 16956823

Keats J.J. et al. 2007. Cancer Cell. 12:131-144. PMID: 17692805

Dib A. et al. 2008. J. Natl. Cancer Inst. Monogr. 39:25-31. PMID: 18647998

Leone et al. 2008. Clin Cancer Res. 14(19):6033-41. PMID: 18829482

Moreaux J. et al. 2011. Haematologica. 96:574-582. PMID: 21173094

Maiga S. et al. 2015. Cytometry A. 87:285-288. PMID: 25688540

Tessoulin B. et al. 2018. J. Hematol. Oncol. 11:137.1-137.13. PMID: 30545397

Sarin V. et al. 2020. Leukemia. 34:2754-2765. PMID: 32123307.

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