TR146 Cell Line

Cell line to investigate buccal mucosa, and as a model for absorption and metabolism of various substances and enzymatically labile drugs.

Contributors

Inventor Institute
Bridget Hill Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151425
Unit size: 1×10^6 cells / vial
Cancer types: Head and neck cancer
Research Fields: Cancer;Drug development;Metabolism
Organism: Human
Tissue: Buccal mucosa that had infiltrated a lymph node
Gender: Female
Model: Tumourigenic cell line
Growth properties: Adherent
Cancer Types In Detail: Human neck metastasis
Primary citation: Rupniak et al. 1985. J Natl Cancer Inst. 75(4):621-35. PMID: 2413234
Alternate name: Tr146; TR-146; TR 146
Product description: Derived from well-differentiated keratinising squamous cell carcinoma of the buccal mucosa the TR146 cell line represents an in vitro model to study permeability, absorption and metabolism of various enzymatically labile drugs and substances including epithelial-mesenchymal transition (EMT) markers, tight junction proteins and aquaporins. Due to morphological similarities and comparable permeability, excised porcine buccal mucosa has been considered a reasonably good model of human buccal mucosa for drug delivery studies. However, for rapid and efficient screening of drug permeability, a cell line generally holds advantages over in vitro models of excised tissue. Closely resembling normal human buccal epithelium, the cell line functions as a reliable oral mucosa model. TR146 has been characterised for permeability of a number of substances of varying molecular weight and hydrophilicity over a range of pH and osmolarity. Activity of aminopeptidase, carboxypeptidase and esterase has also been studied and shown to be comparable with that of human buccal epithelium.
Conditional: Yes
CRISPR: No
Production details: The TR146 cell line originates from a human neck metastasis of a buccal carcinoma, derived from neck node (primary tumour sited in buccal mucosa). Well-differentiated. Female patient, 67 years. Previous radiotherapy (6,000 rads) and neck dissection
Additional notes: Histology: well-differentiated keratinizing squamous cell carcinoma. Cell type: polygonal
Cellosaurus ID: CVCL_2736
Model description: TR146 cell ine is tumourigenic in female (nu/nu) mice resulting in the production of rapidly progressing tumours
disease: Cancer
Gender: Female
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: HAMS F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculture routine: Split sub-confluent cultures (70-80%) seeding at approximately 1-2 x 10^4 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells can reach a maximum density of 7 x 10^4 cells/cm2. Please also see detailed protocol within the Product Datasheet in the Documentation section below.
Temperature: 37°C
Atmosphere: 5% CO2
Mycoplasma free: Yes
Biosafety level: 1
References:

Lin et. al. 2021. Pharmaceutics 13(2):256. PMID: 33673378

Mogavero and Hube. 2021. Methods Mol Biol 2260:133-143. PMID: 33405035

Lin et. al. 2020. Tissue Barriers 8(2): 1784644. PMID: 32614652

Rupel et.al. 2019. NPJ Biofilms Microbiomes. 5: 29. PMID: 31602310

Laurén et.al. 2018. Pharm Res. 22,35(7):145. PMID 29790010

Kalu et al. 2017. Oncotarget. 8(49):86369-86383. PMID: 29156801

Li et al. 2017. Cancer Cell. 31(2):225-239. PMID: 28196595

Pistone et al. 2016. Eur J Pharm Sci. 96:381-389. PMID: 27721043

Nilsen et al. 2016. Eur J Oral Sci. :. PMID: 27711994

Iyire et al. 2016. Sci Rep. 6:32498. PMID: 27581177

Goyer et al. 2016. PLoS One. 11(3):e0149159. PMID: 26933885

Hyakusoku et al. 2016. J Exp Clin Cancer Res. 35:6. PMID: 26754630

Zeng et al. 2015. Int J Pharm. 495(2):1028-37. PMID: 26403384

De Ryck et al. 2015. AMB Express. 5:27. PMID: 25995981

O'Callaghan et al. 2015. Mol Med Rep. :. PMID: 26005189

Chai et al. 2012. J R Soc Interface. 9(77):3528-38. PMID: 22915635

Mrck Nielsen et al. 2000. Int J Pharm. 200(2):261-70. PMID: 10867256

Nielsen et al. 2000. Int J Pharm. 194(2):155-67. PMID: 10692640

Jacobsen et al. 1999. Eur J Pharm Biopharm. 48(3):217-24. PMID: 10612032

Nielsen et al. 1999. Int J Pharm. 185(2):215-25. PMID: 10460917

Nielsen et al. 1999. J Control Release. 60(2-3):223-33. PMID: 10425328

Pedersen et al. 1999. Drug Dev Ind Pharm. 25(4):463-70. PMID: 10194601

Jacobsen et al. 1999. Eur J Oral Sci. 107(2):138-46. PMID: 10232463

Rupniak et al. 1985. J Natl Cancer Inst. 75(4):621-35. PMID: 2413234

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