#151629

MEF Raf1 FF/FF KI Cell Line

Cat. #151629

MEF Raf1 FF/FF KI Cell Line

Cat. #: 151629

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Mouse

Tissue: Embryo

Disease: Cancer

Model: Knock-In

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Catrin Pritchard

Institute: University of Leicester

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: MEF Raf1 FF/FF KI Cell Line
  • Alternate name: Raf-1FF/FF MEF; MEF Raf1 FF/FF KI
  • Research fields: Cancer;Cell signaling and signal transduction;Drug development;Metabolism
  • Parental cell: See the Raf KI FF knockout mouse that these cells were derived from in related products.
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer
  • Model: Knock-In
  • Conditional: Yes
  • Description: The MEF Raf1 FF/FF KI Cell Line is a MEF line obtained from the knock-in mouse carrying inactive Raf-1FF/FF mutant.
  • Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4Â?‚°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
  • Biosafety level: 1
  • Cellosaurus id: CVCL_6D90

Target Details

  • Target: Raf1 Y340F Y341F inactive mutant

Applications

  • Application notes: See the Raf KI FF knockout mouse that these cells were derived from in related products.

Handling

  • Format: Frozen
  • Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
  • Mycoplasma free: Yes

Related Tools

  • Related tools: Raf-1FF/FF knockin mouse

References

  • Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
  • MEK kinase activity is not necessary for Raf-1 function.