MEF Raf1 FF/FF KI Cell Line

The MEF Raf1 FF/FF KI Cell Line is a MEF line obtained from the knock-in mouse carrying inactive Raf-1FF/FF mutant.

Contributors

Inventor Institute
Catrin Pritchard University of Leicester
Cat. #: 151629
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Cell signaling and signal transduction;Drug development;Metabolism
Organism: Mouse
Tissue: Embryo
Model: Knock-In
Alternate name: Raf-1FF/FF MEF; MEF Raf1 FF/FF KI
Product description: The MEF Raf1 FF/FF KI Cell Line is a MEF line obtained from the knock-in mouse carrying inactive Raf-1FF/FF mutant.
Conditional: Yes
Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4Â?‚°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
Cellosaurus ID: CVCL_6D90
Parental cell line: See the Raf KI FF knockout mouse that these cells were derived from in related products.
disease: Cancer
Format: Frozen
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry ice
Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
Mycoplasma free: Yes
Biosafety level: 1
References:

Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.

MEK kinase activity is not necessary for Raf-1 function.

Images

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MEF Raf1 FF/FF KI Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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