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#151232

Anti-Cdc27 [AF 3.1]

Cat. #151232

Anti-Cdc27 [AF 3.1]

Cat. #: 151232

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: Cell division cycle protein 27 (cdc27)

Class: Monoclonal

Application: ELISA ; FACS ; IHC ; IP ; WB

Reactivity: Mammalian ; Xenopus laevis

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Julian Gannon

Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-Cdc27 [AF 3.1]
  • Alternate name: Cell Division Cycle 27; Anaphase Promoting Complex Subunit; DS143E; D17S978E; CDC27Hs; ANAPC3; H-NUC; APC3; Anaphase-Promoting Complex; Cell Division Cycle 27 Homolog; CDC27 Homolog; Nuc2 Homolog; HNUC; NUC2
  • Research fields: Biochemistry;Cell biology
  • Clone: AF 3.1
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 80 kDa
  • Strain: Balb/c
  • Reactivity: Mammalian ; Xenopus laevis
  • Host: Mouse
  • Application: ELISA ; FACS ; IHC ; IP ; WB
  • Description: AF 3.1 is recommended for the immunoprecipitation of active APC.
  • Immunogen: A peptide corresponding to the C-terminal 10 residues of human cdc27 coupled to KLH.
  • Isotype: IgG2b
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: HeLa cells

Target Details

  • Target: Cell division cycle protein 27 (cdc27)
  • Molecular weight: 80 kDa
  • Tissue cell line specificity: HeLa cells
  • Target background: Cdc27 is a component of the anaphase promoting complex (APC) which is responsible for destroying proteins, including cyclins, which are involved in mitosis.

Applications

  • Application: ELISA ; FACS ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Dry ice

References

  • Mateo F et al. Oncogene. 2009 Jul 23
  • 28(29):2654-66.
  • Ahlskog et al. 2010. Mol Cell Biol. 30(24):5608-20. PMID: 20937767.
  • Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress.
  • Holt et al. 2010. Development. 137(8):1297-304. PMID: 20223764.
  • Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes.
  • Hochegger et al. 2007. J Cell Biol. 178(2):257-68. PMID: 17635936.
  • An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells.
  • Yamano et al. 1998. EMBO J. 17(19):5670-8. PMID: 9755167.
  • The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor.

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