#151631

C-Raf KI D486A Cell Line

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Cat. #151631

C-Raf KI D486A Cell Line

Cat. #: 151631

Unit size: 1x10^6 cells / vial

Organism: Mouse

Tissue: Embryo

Disease: Cancer

Model: Knock-In

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Catrin Pritchard

Institute: University of Leicester

Tool Details
Target Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: C-Raf KI D486A Cell Line
  • Research fields: Cancer;Cell signaling and signal transduction;Drug development
  • Parental cell: See the Raf-1 D486A oncogenic mutant knockin mouse that these cells are produced from.
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer
  • Model: Knock-In
  • Description: The C-Raf KI D486A Cell Line is derived from the knock-in mouse model carrying oncogenic Raf-1 D486A; it is useful for in vivo study of oncogenic Raf-1 mutants and Ras signalling. See the Raf-1 D486A oncogenic mutant knockin mouse that these cells are produced from.
  • Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4Â?‚°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.

Target Details

  • Target: Raf1 D486A oncogenic mutant

Handling

  • Format: Frozen
  • Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

Related Tools

  • Related tools: Raf-1 D486A oncogenic mutant knockin mouse

References

  • Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.
  • CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.