C-Raf KI D486A Cell Line

The C-Raf KI D486A Cell Line is derived from the knock-in mouse model carrying oncogenic Raf-1 D486A; it is useful for in vivo study of oncogenic Raf-1 mutants and Ras signalling.

Contributors

Inventor Institute
Catrin Pritchard University of Leicester
Cat. #: 151631
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Cell signaling and signal transduction;Drug development
Organism: Mouse
Tissue: Embryo
Model: Knock-In
Product description: The C-Raf KI D486A Cell Line is derived from the knock-in mouse model carrying oncogenic Raf-1 D486A; it is useful for in vivo study of oncogenic Raf-1 mutants and Ras signalling. See the Raf-1 D486A oncogenic mutant knockin mouse that these cells are produced from.
Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
Parental cell line: See the Raf-1 D486A oncogenic mutant knockin mouse that these cells are produced from.
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
References:

Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.

CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.

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