V143.1 is exceptionally good at detecting Cyclin B1 in western blots, giving a very strong signal with minimal background.
| Inventor | Institute |
|---|---|
| Julian Gannon | Cancer Research UK, London Research Institute: Clare Hall Laboratories |
| Cat. #: | 151020 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Cancer;Cell biology;Genetics |
| Application: | WB |
| Target: | Cyclin B1 |
| Reactivity: | Mouse |
| Clone: | V143.1 |
| Host: | Mouse |
| Class: | Monoclonal |
| Alternate name: | CCNB1; Cyclin B1; G2/Mitotic-Specific Cyclin B1; CCNB |
|---|---|
| Product description: | V143.1 is exceptionally good at detecting Cyclin B1 in western blots, giving a very strong signal with minimal background. |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Immunogen: | His tagged cyclin B1 (hamster) expressed in bacteria. |
| Myeloma used: | Sp2/0-Ag14 |
| Target background: | Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin B1 is a marker of cell proliferation and a key component of the cell cycle progression machinery. Cyclin B1 may prove to be a prognostic marker in neoplasia. |
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| Format: | Liquid |
|---|---|
| Concentration: | 0.9-1.1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | '-15° C to -25° C |
| Shipping conditions: | Dry ice |
| References: |
Shen et al. 2010. Cell Cycle. 9(23):4720-34. PMID: 21099356. Female infertility in PDE3A(-/-) mice: polo-like kinase 1 (Plk1) may be a target of protein kinase A (PKA) and involved in meiotic arrest of oocytes from PDE3A(-/-) mice. |
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