Cat. #151554
SAP1 Mouse
Cat. #: 151554
Sub-type: Mouse
Availability: 8-10 weeks
Disease: Castleman disease
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Richard Treisman
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: SAP1 Mouse
- Research fields: Cell signaling and signal transduction;Genetics;Immunology
- Tool sub type: Mouse
- Disease: Castleman disease
- Conditional: No
- Description: In vivo study of SAP-1 knockout; in vivo study of positive T cell selection & development
- Genetic background: An Elk4 targeting vector, containing a resistance cassette inserted between exons 1 and 4, was transfected into 129 ES cells. Properly targeted ES cells containing a homologus recombination event were selected, cloned and injected into blastocysts. Chimeric offspring were backcrossed to establish heterozygous lines, and interbred to establish homozygous mice.
- Phenotype: The SAP-1 mouse demonstrate a phenotype analogous to mutlicentric Castleman disease when maintained in non-sterile housing.SAP-1 mice exhibit abnormalities in T cell populations, resulting from abnormal negative selection during T cell development.
- Production details: An Elk4 targeting vector, containing a resistance cassette inserted between exons 1 and 4, was transfected into 129 ES cells. Properly targeted ES cells containing a homologus recombination event were selected, cloned and injected into blastocysts. Chimeric offspring were backcrossed to establish heterozygous lines, and interbred to establish homozygous mice.
- Additional notes: The SAP-1 mouse demonstrate a phenotype analogous to mutlicentric Castleman disease when maintained in non-sterile housing.SAP-1 mice exhibit abnormalities in T cell populations, resulting from abnormal negative selection during T cell development.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Elk4 / SAP-1
References
- Costello et al. 2004. Nat Immunol. 5(3):289-98. PMID: 14770179.
- Ternary complex factor SAP-1 is required for Erk-mediated thymocyte positive selection.
