Cat. #151467
p23+/- Mouse
Cat. #: 151467
Sub-type: Mouse
Availability: 8-10 weeks
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Mike Owen
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: p23+/- Mouse
- Research fields: Cancer;Cell signaling and signal transduction;Genetics;Immunology
- Tool sub type: Mouse
- Description: In vivo study of p23 knockout, golgi apparatus and early secretory pathway function
- Genetic background: A p23 targeting vector, constructed from genomic fragments, but replacing exon 1 of the p23 gene with a resistance marker, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the p23 knockout allele, p23+/- mice.
- Phenotype: Golgi apparatus abnormalities
- Zygosity: Heterozygous
- Strain: 129/C57BL/6
- Production details: A p23 targeting vector, constructed from genomic fragments, but replacing exon 1 of the p23 gene with a resistance marker, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the p23 knockout allele, p23+/- mice.
- Additional notes: Genetic Bkg: 129/C57BL/6. Zygosity: Heterozygous
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: p23
References
- Denzel et al. 2000. Curr Biol. 10(1):55-8. PMID: 10660306.
- The p24 family member p23 is required for early embryonic development.
