#151626

A375P beta6-puro Cell Line

Please Enter Your Email ID
Email ID

Cat. #151626

A375P beta6-puro Cell Line

Cat. #: 151626

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-5 days

Organism: Human

Disease: Cancer

Model: Transgenic

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: John Marshall

Institute: Queen Mary University of London

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: A375P beta6-puro Cell Line
  • Cancer: Skin cancer
  • Cancers detailed: Melanoma
  • Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Drug development
  • Tool sub type: Continuous
  • Parental cell: A375P
  • Organism: Human
  • Disease: Cancer
  • Growth properties: A375P melanoma cells stably expressing integrin beta 6
  • Model: Transgenic
  • Description: The A375P beta6-puro Cell Line is a useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent A375 line. A375 melanoma cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of AvB6-binding peptides.
  • Production details: The human melanoma cell line A375P was infected with pBabe retroviruses encoding puromycin resistance or, in addition, cDNA for human integrin beta 6. Cells were selected in puromycin (1.25 ug/mL) followed by magnetic bead sorting, using 10D5 (mouse anti-avh6; Chemicon International), according to the manufacturerâ?‚€?‚™s instructions (Dynal; Invitrogen). The avb6 expression can drift so recipients must check by flow cytometery after they have been in continuous passage, at least once a ...
  • Biosafety level: 1
  • Recommended controls: A375P parental line
  • Cellosaurus id: CVCL_5F66

Target Details

  • Target: Integrin beta 6

Handling

  • Format: Frozen
  • Growth medium: DMEM + 10S/1.25ug/ml puromycin. Antibotic is not required all the time.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
  • Mycoplasma free: Yes

References

  • DiCara et al. 2007. J Biol Chem. 282(13):9657-65. PMID: 17244604.
  • Structure-function analysis of Arg-Gly-Asp helix motifs in alpha v beta 6 integrin ligands.