A375P beta6-puro Cell Line

The A375P beta6-puro Cell Line is a useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent A375 line. A375 melanoma cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of AvB6-binding peptides.

Contributors

Inventor Institute
John Marshall Queen Mary University of London
Cat. #: 151626
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Skin cancer
Research Fields: Cancer;Cell biology;Cell signaling and signal transduction;Drug development
Organism: Human
Model: Transgenic
Growth properties: A375P melanoma cells stably expressing integrin beta 6
Cancer Types In Detail: Melanoma
Product description: The A375P beta6-puro Cell Line is a useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent A375 line. A375 melanoma cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of AvB6-binding peptides.
Production details: The human melanoma cell line A375P was infected with pBabe retroviruses encoding puromycin resistance or, in addition, cDNA for human integrin beta 6. Cells were selected in puromycin (1.25 ug/mL) followed by magnetic bead sorting, using 10D5 (mouse anti-avh6; Chemicon International), according to the manufacturerâ?‚€?‚™s instructions (Dynal; Invitrogen). The avb6 expression can drift so recipients must check by flow cytometery after they have been in continuous passage, at least once a …
Cellosaurus ID: CVCL_5F66
Parental cell line: A375P
disease: Cancer
Format: Frozen
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry ice
Growth medium: DMEM + 10S/1.25ug/ml puromycin. Antibotic is not required all the time.
Recommended controls: A375P parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

DiCara et al. 2007. J Biol Chem. 282(13):9657-65. PMID: 17244604.

Structure-function analysis of Arg-Gly-Asp helix motifs in alpha v beta 6 integrin ligands.

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