#151663

MEF PKCe KO KI Cell Line

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Cat. #151663

MEF PKCe KO KI Cell Line

Cat. #: 151663

Unit size: 1x10^6 cells / vial

Organism: Mouse

Tissue: Embryo

Disease: Cancer

Model: Transgenic

£575.00

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Contributor

Inventor: Peter Parker

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MEF PKCe KO KI Cell Line
  • Research fields: Cancer;Cell biology;Cell signaling and signal transduction
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer
  • Growth properties: Normal phenotype
  • Model: Transgenic
  • Description: MEF PKCe KO KI Cell Line is a clonal MEF line derived from PKCepsilon knock-out embryos subsequently transfected to re-express PKCe through stable transfection with pcDNA3 CMV/IE hygo+ PKCe. Matched isogenic cell line which is null for PKCe is also available. Useful for studying the role of PKCe in various biological processes
  • Production details: MEFs were derived from PKCe knockout embryos. Polyclonal PKCeKO cells were transfected with pcDNA3 CMV/IE hygro+ PKCe vector using calcium phosphate. Clonal stable cell lines were selected using limiting dilution. The vector was contructed by replacing the promoter region in pCMV hygro+ with a 2.1kb CMV/IE promoter derived from p63d. Mouse cDNA encoding full-length PKCe (3.3kb fragment) was ligated to the modified pcDNA3 CMV/IE hygro+ vector.
  • Cellosaurus id: CVCL_AS82

Target Details

  • Target: PKC epsilon

Handling

  • Format: Frozen
  • Growth medium: Cells were selected, and are routinely cultured in DMEM + 10% FCS and 100ug/ml hygromycin at 30degC in a 10% CO2 atmosphere
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Cited by 24 subsequent publications including:
  • Ivaska, J. et al. 2002. EMBO J. 21:3608-19.PMID: 12110574
  • Kermorgant, S. et al. 2004. EMBO J. 23: 3721:34. PMID 15385963
  • Pardo et al. 2006. EMBO J. 25(13):3078-88. PMID: 16810323.
  • FGF-2 protects small cell lung cancer cells from apoptosis through a complex involving PKCepsilon, B-Raf and S6K2.