MEF PKCe KO KI Cell Line

MEF PKCe KO KI Cell Line is a clonal MEF line derived from PKCepsilon knock-out embryos subsequently transfected to re-express PKCe through stable transfection with pcDNA3 CMV/IE hygo+ PKCe.

Contributors

Inventor Institute
Peter Parker Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151663
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Cell biology;Cell signaling and signal transduction
Organism: Mouse
Tissue: Embryo
Model: Transgenic
Growth properties: Normal phenotype
Product description: MEF PKCe KO KI Cell Line is a clonal MEF line derived from PKCepsilon knock-out embryos subsequently transfected to re-express PKCe through stable transfection with pcDNA3 CMV/IE hygo+ PKCe. Matched isogenic cell line which is null for PKCe is also available. Useful for studying the role of PKCe in various biological processes
Production details: MEFs were derived from PKCe knockout embryos. Polyclonal PKCeKO cells were transfected with pcDNA3 CMV/IE hygro+ PKCe vector using calcium phosphate. Clonal stable cell lines were selected using limiting dilution. The vector was contructed by replacing the promoter region in pCMV hygro+ with a 2.1kb CMV/IE promoter derived from p63d. Mouse cDNA encoding full-length PKCe (3.3kb fragment) was ligated to the modified pcDNA3 CMV/IE hygro+ vector.
Cellosaurus ID: CVCL_AS82
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Cells were selected, and are routinely cultured in DMEM + 10% FCS and 100ug/ml hygromycin at 30degC in a 10% CO2 atmosphere
References:

Cited by 24 subsequent publications including:

Ivaska, J. et al. 2002. EMBO J. 21:3608-19.PMID: 12110574

Kermorgant, S. et al. 2004. EMBO J. 23: 3721:34. PMID 15385963

Pardo et al. 2006. EMBO J. 25(13):3078-88. PMID: 16810323.

FGF-2 protects small cell lung cancer cells from apoptosis through a complex involving PKCepsilon, B-Raf and S6K2.

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