Cat. #156524
MCF10A pLA(empty) (negCTRL for hRas_V12 cell line)
Cat. #: 156524
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Tissue: Breast
Disease: Cancer
Model: Cancer Model
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Sarah-Maria Fendt
Institute: Vlaams Instituut voor Biotechnologie (VIB)
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: MCF10A pLA(empty) (negCTRL for hRas_V12 cell line)
- Alternate name: transforming protein p21, h-Ras, H-Ras, HRAS
- Research fields: Cancer
- Tool sub type: Continuous
- Parental cell: MCF10A
- Tissue: Breast
- Disease: Cancer
- Model: Cancer Model
- Description: MCF10A cells are frequently used to study signalling pathways in breast cancer. They can be grown in 3D culture. The addition of the H-Ras12 simulates breast cancer as 50% of breast cancers display increased H-Ras activity. This is a control line for the MCF10 H-Ras12 cell line from the same group at the VIB as it has the same pLA expression vector stably expressed but lacking the HRAS gene.
- Biosafety level: 1
- Additional notes: This cell line is a negative control for the cell line overexpressing HRas. It was made using the same pLA vector but lacking the HRAS gene.
Target Details
- Target: HRas
Applications
- Application notes: This cell line is a negative control for the cell line overexpressing HRas. It was made using the same pLA vector but lacking the HRAS gene.
Handling
- Format: Frozen
- Growth medium: DMEM-F12 supplemented with 5% horse serum, 1% penicillin (50 units ml-1), 1% streptomycin (50 ?g ml-1), 0.5 ?g ml-1 hydrocortisone, 100 ng ml-1 cholera toxin, 10 ?g ml-1 insulin and 20 ng ml-1 recombinant human EGF.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
Related Tools
- Related tools: MCF10A hRas_V12 cell line
References
- Elia et al. 2017. Nat Commun. 8:15267. PMID: 28492237.
