MCF10A pLA(empty) (negCTRL for hRas_V12 cell line)

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: MCF10A pLA(empty) (negCTRL for hRas_V12 cell line)
• Alternate name: transforming protein p21, h-Ras, H-Ras, HRAS
• Research fields: Cancer
• Tool sub type: Continuous
• Parental cell: MCF10A
• Tissue: Breast
• Disease: Cancer
• Model: Cancer Model
• Description: MCF10A cells are frequently used to study signalling pathways in breast cancer. They can be grown in 3D culture. The addition of the H-Ras12 simulates breast cancer as 50% of breast cancers display increased H-Ras activity. This is a control line for the MCF10 H-Ras12 cell line from the same group at the VIB as it has the same pLA expression vector stably expressed but lacking the HRAS gene.
• Biosafety level: 1
• Additional notes: This cell line is a negative control for the cell line overexpressing HRas. It was made using the same pLA vector but lacking the HRAS gene.

Target Details

• Target: HRas

Applications

• Application notes: This cell line is a negative control for the cell line overexpressing HRas. It was made using the same pLA vector but lacking the HRAS gene.

Handling

• Format: Frozen
• Growth medium: DMEM-F12 supplemented with 5% horse serum, 1% penicillin (50 units ml-1), 1% streptomycin (50 ?g ml-1), 0.5 ?g ml-1 hydrocortisone, 100 ng ml-1 cholera toxin, 10 ?g ml-1 insulin and 20 ng ml-1 recombinant human EGF.
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

Related Tools

• Related tools: MCF10A hRas_V12 cell line

References

• Elia et al. 2017. Nat Commun. 8:15267. PMID: 28492237.