CMT 64 Cell Line

CMT 64 murine cell line is an in vivo mouse tumourigenesis system to study the growth characteristics and metastasis of mouse tumour lines.

Contributors

Inventor Institute
Peter Riddle Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151447
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Organism: Mouse
Tissue: Lung
Gender: Female
Model: Cancer cell line
Growth properties: Adherent
Primary citation: Franks et al. 1976. Cancer Res. 36(3):1049-1055. PMID: 1253168.
Alternate name: CMT-64; CMT.64; CMT64; C57 Mouse Tumor 64
Product description: The tumourigenic and metastatic CMT 64 murine cell line is an in vivo mouse tumourigenesis system to study the growth characteristics and metastasis of mouse tumour lines (homoplastic with stable CMT 167 line); it demonstrates stable growth characteristics and morphology in culture and in lung metastasis induced after subcutaneous inoculation of mice. This demonstrates its consistency and reliability as a model for studying lung carcinoma progression and metastasis.
Gender: Female
CRISPR: Yes
Conditional: Yes
Production details: CMT 64 was isolated from a primary alveogenic lung carcinoma tumour in a C57BL/1CRF mouse. The cell line maintained a stable morphology and growth rate comparable to that observed in the original tumour tissue in vitro. Notably, CMT 64 retained similar characteristics in both primary tumour growth and in lung metastases following subcutaneous inoculation in mice.
Cellosaurus ID: CVCL_2406
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10^4 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density 1.2 x 10^5 cells/cm². Please also see detailed protocol within the Product Datasheet in the Documentation section below.
Mycoplasma free: Yes
References:

Miyashita N et al. 2021. Sci Rep. 17:11(1):22380. PMID: 34789779

Rincon et al. 2017. Oncotarget. : PMID: 28525366

Franks et al. 1976. Cancer Res. 36(3):1049-1055. PMID: 1253168

Images

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CMT 64 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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