#161706

ER-Hoxb8 cells WASP+/+ with cell surface antigens T.Y-1.1

Cat. #161706

ER-Hoxb8 cells WASP+/+ with cell surface antigens T.Y-1.1

Cat. #: 161706

Sub-type: Continuous

Organism: Mouse

Tissue: Bone Marrow

Disease: Blood disorders

Model: Knock-Out

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Véronique Le Cabec, Thibaut Sanchez, Frederic Lagarrigue

Institute: Toulouse Tech Transfer

Primary Citation: Accarias et al. 2020. J Cell Sci, 133 (5): jcs236703, PMID: 31964707

Tool Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: ER-Hoxb8 cells WASP+/+ with cell surface antigens T.Y-1.1
  • Alternate name: WASP deficient haematopoietic precursors with cell surface antigens TY-1.1
  • Tool sub type: Continuous
  • Organism: Mouse
  • Tissue: Bone Marrow
  • Disease: Blood disorders
  • Morphology: Variable amoeboid, elongated spindle-like, or round
  • Growth properties: Mixed - adherent and suspension
  • Model: Knock-Out
  • Model description: WASP deficient haematopoietic precursors using CRISPR/Cas9
  • Crispr: Yes
  • Conditional: Yes
  • Conditional description: Conditional HoxB8 expression under ER promoter enabling transcriptional activation to produce immortalized factor-dependant progenitors
  • Description: Immortalised ER-Hoxb8 bone marrow progenitor cells expressing cell surface antigens TY-1.1 used to study macrophage functions with implications in cancer immunotherapies targeting the motility of tumour-associated macrophages
  • Application: Scanning electron microscopy, FACS, Fluorescence, Immunofluorescence
  • Production details: To generate the ER-Hoxb8 Wasp?/? cells, 106 ER-Hoxb8 progenitor cells were seeded on fibronectin coated six-well culture plates in myeloid medium and transduced with 2?ml of viral suspension (as described above) by spinoculation (1000?g, 90?min, 22°C) in the presence of polybrene (8??g/ml). Polybrene was serially diluted by renewing half of the medium over several days to obtain a final concentration of 1.4??g/ml. Antibiotic selection of transduced ER-Hoxb8 cells was performed 3?days post-inf...
  • Biosafety level: 2
  • Recommended controls: ER-Hoxb8 cells WASP+/+ without cell surface antigens T.Y-1.1

Applications

  • Application: Scanning electron microscopy, FACS, Fluorescence, Immunofluorescence

Handling

  • Growth medium: Progenitor Medium: RPMI w/ Glutamax (Gibco 61870044), 10% Heat Inactivated FBS, 1x Antibiotic / Antimycotic (Gibco 15240062), 20 ng/mL GM-CSF (Miltenyi 130-095-735), 0.5 ?M ?-Estradiol (Sigma-Aldrich E2758-250MG).
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Subculture routine: If medium is exhausted, spin at RT for 5 min at 300g. Discard medium and resuspend cells in fresh medium. On Mondays and Wednesdays: seed 1.105 cells/mL in new progenitor medium. On Fridays: seed 5.104 cells/mL in new progenitor medium.
  • Cultured in antibiotics: Gibco™ Antibiotic-Antimycotic

Related Tools

  • Related tools: ER-Hoxb8 cells WASP+/+ without cell surface antigens T.Y-1.1

References

  • Accarias et al. 2020. J Cell Sci, 133 (5): jcs236703, PMID: 31964707