Cat. #153332
CMT 170 Cell Line
Cat. #: 153332
Unit size: 1x10^6 cells / vial
Organism: Mouse
Tissue: Lung
Disease: Cancer
Model: Cancer cell line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Leonard Franks
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: CMT 170 Cell Line
- Cancer type filter: Lung cancer
- Cancers detailed: Lung carcinoma;Metastatic
- Research fields: Cancer
- Organism: Mouse
- Gender: Female
- Tissue: Lung
- Disease: Cancer
- Growth properties: Adherent
- Model: Cancer cell line
- Description: CMT 170 is a highly metastatic murine alveogenic lung carcinoma cell line, derived from the parental CMT 64 line. Developed in 1984 at the Cancer Research UK London Research Institute (Lincoln's Inn Fields), CMT 170 was established through subcloning and in vivo selection for enhanced metastatic potential from pooled lung metastases of CMT 64. Compared to CMT 64, CMT 170 displays increased metastasis and is a useful tool to investigate the mechanisms and origins of metastatic progression in lung cancer.
- Production details: To generate CMT 170, adult female C57B/T mice (aged 4–6 months) were subcutaneously injected with CMT 167 culture passage number 4 cells (generated from the parental CMT 64 line). Following tumour development, cells were harvested from resulting lung metastases. Epithelial outgrowths from these metastatic sites were cultured and selectively expanded based on their high metastatic potential. Through this process of subcloning and in vivo screening, the CMT 170 cell line was established as a more aggressively metastatic variant of CMT 64.
- Cellosaurus id: CVCL_B492
Handling
- Format: Frozen
- Growth medium: DMEM with 2mM Glutamine and 10% FCS.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Please also see detailed protocol within the Product Datasheet in the Documentation section below.
Related Tools
- Related tools: CMT 64 Cell Line
References
- Franks et al. 1984. Br J Cancer. 49(4):423-9. PMID: 6324837
