1-7 CE1 Cell Line

The human mammary luminal epithelial cell line MTSV1-7 was transfected with an ERBB2 expression vector, enabling over-expression of the ERBB2 proto-oncogene

Contributors

Inventor Institute
Joyce Taylor-Papadimitriou ; Joy Burchell Cancer Research UK, Lincoln's Inn Fields Institute
Cat. #: 151503
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Cell biology;Cell signaling and signal transduction;Drug development
Organism: Human
Tissue: Breast
Gender: Female
Model: Transgenic
Cancer Types In Detail: Breast cancer
Product description: 1-7 CE1 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7. MTSV1-7 is transfected with erb-b2 receptor tyrosine kinase 2 (ERBB2) expression vector, enabling the over-expression of ERBB2 proto-oncogene. At confluence, the morphology is cuboidal. The ERBB2 (or Her2) oncogene is amplified and/or overexpressed in a significant proportion of breast cancers. The over-expression of the ERBB2 receptor inhibits morphogenesis in vitro, and induces reduced expression of surface adhesion molecules such as E-cadherin, suggesting a role for ERBB2 over-expression in tumour progression and metastasis. The 1-7 CE1 cell line is an ideal in vitro tool for investigation of the role of the ERBB2 proto-oncogene in breast cancer morphogenesis and adhesion.
Gender: Female
Production details: MTSV1-7 transfected with SV40 plasmid DNA; MTSV1-7 ce1 line transfected with plasmid constructs for pSV2-erbB2 and pSV2neo.
Cellosaurus ID: CVCL_3033
Parental cell line: MSTV1-7 HER2
disease: Cancer
Format: Frozen
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry ice
Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + Insulin (bovine) at 10ug/ml + Hydrocortisone at 5ug/ml.
Temperature: 37° C
Atmosphere: 5% CO2
Recommended controls: MSTV1-7 HER2 parental line
References:

D'souza et al. 1994. Proc Natl Acad Sci U S A. 91(15):7202-7206. PMID: 7913748.

D'Souza et al. 1993. Oncogene. 8(7):1797-1806. PMID: 8099725.

Bartek et al. 1991. Proc Natl Acad Sci U S A. 88(9):3520-3524. PMID: 1708884.

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