#151503

1-7 CE1 Cell Line

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Cat. #151503

1-7 CE1 Cell Line

Cat. #: 151503

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Organism: Human

Tissue: Breast

Disease: Cancer

Model: Transgenic

£575.00

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Contributor

Inventor: Joyce Taylor-Papadimitriou ; Joy Burchell

Institute: Cancer Research UK, Lincoln's Inn Fields Institute

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: 1-7 CE1 Cell Line
  • Cancer: Breast cancer
  • Cancers detailed: Breast cancer
  • Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Drug development
  • Tool sub type: Continuous
  • Parental cell: MSTV1-7 HER2
  • Organism: Human
  • Gender: Female
  • Tissue: Breast
  • Disease: Cancer
  • Model: Transgenic
  • Description: 1-7 CE1 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7. MTSV1-7 is transfected with erb-b2 receptor tyrosine kinase 2 (ERBB2) expression vector, enabling the over-expression of ERBB2 proto-oncogene. At confluence, the morphology is cuboidal. The ERBB2 (or Her2) oncogene is amplified and/or overexpressed in a significant proportion of breast cancers. The over-expression of the ERBB2 receptor inhibits morphogenesis in vitro, and induces reduced expression of surface adhesion molecules such as E-cadherin, suggesting a role for ERBB2 over-expression in tumour progression and metastasis. The 1-7 CE1 cell line is an ideal in vitro tool for investigation of the role of the ERBB2 proto-oncogene in breast cancer morphogenesis and adhesion.
  • Application: Investigation of ERBB2 proto-oncogene in breast cancer
  • Production details: MTSV1-7 transfected with SV40 plasmid DNA; MTSV1-7 ce1 line transfected with plasmid constructs for pSV2-erbB2 and pSV2neo.
  • Recommended controls: MSTV1-7 HER2 parental line
  • Cellosaurus id: CVCL_3033

Target Details

  • Target: ERBB2

Applications

  • Application: Investigation of ERBB2 proto-oncogene in breast cancer

Handling

  • Format: Frozen
  • Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + Insulin (bovine) at 10ug/ml + Hydrocortisone at 5ug/ml.
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.

Related Tools

  • Related tools: 1-7HB2 Cell Line

References

  • D'souza et al. 1994. Proc Natl Acad Sci U S A. 91(15):7202-7206. PMID: 7913748.
  • D'Souza et al. 1993. Oncogene. 8(7):1797-1806. PMID: 8099725.
  • Bartek et al. 1991. Proc Natl Acad Sci U S A. 88(9):3520-3524. PMID: 1708884.