Cat. #151503
1-7 CE1 Cell Line
Cat. #: 151503
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Breast
Disease: Cancer
Model: Transgenic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Joyce Taylor-Papadimitriou ; Joy Burchell
Institute: Cancer Research UK, Lincoln's Inn Fields Institute
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: 1-7 CE1 Cell Line
- Cancer: Breast cancer
- Cancers detailed: Breast cancer
- Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Drug development
- Tool sub type: Continuous
- Parental cell: MSTV1-7 HER2
- Organism: Human
- Gender: Female
- Tissue: Breast
- Disease: Cancer
- Model: Transgenic
- Description: 1-7 CE1 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7. MTSV1-7 is transfected with erb-b2 receptor tyrosine kinase 2 (ERBB2) expression vector, enabling the over-expression of ERBB2 proto-oncogene. At confluence, the morphology is cuboidal. The ERBB2 (or Her2) oncogene is amplified and/or overexpressed in a significant proportion of breast cancers. The over-expression of the ERBB2 receptor inhibits morphogenesis in vitro, and induces reduced expression of surface adhesion molecules such as E-cadherin, suggesting a role for ERBB2 over-expression in tumour progression and metastasis. The 1-7 CE1 cell line is an ideal in vitro tool for investigation of the role of the ERBB2 proto-oncogene in breast cancer morphogenesis and adhesion.
- Application: Investigation of ERBB2 proto-oncogene in breast cancer
- Production details: MTSV1-7 transfected with SV40 plasmid DNA; MTSV1-7 ce1 line transfected with plasmid constructs for pSV2-erbB2 and pSV2neo.
- Recommended controls: MSTV1-7 HER2 parental line
- Cellosaurus id: CVCL_3033
Target Details
- Target: ERBB2
Applications
- Application: Investigation of ERBB2 proto-oncogene in breast cancer
Handling
- Format: Frozen
- Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + Insulin (bovine) at 10ug/ml + Hydrocortisone at 5ug/ml.
- Temperature: 37° C
- Atmosphere: 5% CO2
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Related Tools
- Related tools: 1-7HB2 Cell Line
References
- D'souza et al. 1994. Proc Natl Acad Sci U S A. 91(15):7202-7206. PMID: 7913748.
- D'Souza et al. 1993. Oncogene. 8(7):1797-1806. PMID: 8099725.
- Bartek et al. 1991. Proc Natl Acad Sci U S A. 88(9):3520-3524. PMID: 1708884.
