1-7 CE1 Cell Line
Exclusive toolThe human mammary luminal epithelial cell line MTSV1-7 was transfected with an ERBB2 expression vector, enabling over-expression of the ERBB2 proto-oncogene
Contributors
| Inventor | Institute |
|---|---|
| Joyce Taylor-Papadimitriou ; Joy Burchell | Cancer Research UK, Lincoln's Inn Fields Institute |
| SKU: | 151503 |
|---|---|
| Product description: | 1-7 CE1 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7. MTSV1-7 is transfected with erb-b2 receptor tyrosine kinase 2 (ERBB2) expression vector, enabling the over-expression of ERBB2 proto-oncogene. At confluence, the morphology is cuboidal. The ERBB2 (or Her2) oncogene is amplified and/or overexpressed in a significant proportion of breast cancers. The over-expression of the ERBB2 receptor inhibits morphogenesis in vitro, and induces reduced expression of surface adhesion molecules such as E-cadherin, suggesting a role for ERBB2 over-expression in tumour progression and metastasis. The 1-7 CE1 cell line is an ideal in vitro tool for investigation of the role of the ERBB2 proto-oncogene in breast cancer morphogenesis and adhesion. |
| Gender: | Female |
| Production details: | MTSV1-7 transfected with SV40 plasmid DNA; MTSV1-7 ce1 line transfected with plasmid constructs for pSV2-erbB2 and pSV2neo. |
| Cellosaurus ID: | CVCL_3033 |
| Parental cell line: | MSTV1-7 HER2 |
| Disease: | Cancer |
| Cat. #: | 151503 |
|---|---|
| Provenance and Ownership: | The 1-7 CE1 Cell Line is owned by Cancer Research UK. CancerTools supplies the 1-7 CE1 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing 1-7 CE1 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Breast cancer |
| Organism: | Human |
| Tissue: | Breast |
| Gender: | Female |
| Model: | Transgenic |
| Cancer Types In Detail: | Breast cancer |
| Format: | Frozen |
|---|---|
| Storage conditions: | Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability. |
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + Insulin (bovine) at 10ug/ml + Hydrocortisone at 5ug/ml. |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 |
| Recommended controls: | MSTV1-7 HER2 parental line |
| References: |
D'souza et al. 1994. Proc Natl Acad Sci U S A. 91(15):7202-7206. PMID: 7913748. D'Souza et al. 1993. Oncogene. 8(7):1797-1806. PMID: 8099725. Bartek et al. 1991. Proc Natl Acad Sci U S A. 88(9):3520-3524. PMID: 1708884. |
|---|
Images
View Gallery
Product Images
MTSV1-7 CE1 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)
Related Tools for Cell lines
| Cat. # | Tool Name | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 151445 | 1-7HB2 Cell Line |
Key Info
1-7HB2 Cell Line
|
View Tool | |||||||||
