Cat. #152751
CMT 64/61 Cell Line
Cat. #: 152751
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Mouse
Tissue: Lung
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Peter Riddle
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: CMT 64/61 Cell Line
- Cancer: Lung cancer
- Cancers detailed: Lung carcinoma;Metastatic
- Research fields: Cancer;Drug development
- Organism: Mouse
- Tissue: Lung
- Disease: Cancer
- Growth properties: Tumorigenesis, adherent
- Model: Tumour line
- Conditional: Yes
- Description: The tumourigenic and metastatic CMT 64/61 murine cell line is derived from an alveologenic lung carcinoma of a C57BL/1CRF mouse. It can be maintained in a completely defined serum free medium, but the growth rate is much slower than in supplemented serum. It has been used in studies of lung metastases.
- Production details: Mouse; CMT 64 isolated from primary alveogenic lung carcinoma tumour mass in C57BL/lcrf mouse, achieving stable morphology and growth rate in culture, similar to growth rate and morphology in primary tumour, and in lung metastasis induced after subcutaneous innoculation of mice.
- Cellosaurus id: CVCL_4146
Handling
- Format: Frozen
- Growth medium: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm? using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37?°C. Culture Medium: Waymouth's MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: CMT 64 Cell Line ; CMT 167 Cell Line
References
- Franks et al. 1976. Cancer Res. 36(3):1049-55. PMID: 1253168.
- Metastasizing tumors from serum-supplemented and serum-free cell lines from a C57BL mouse lung tumor.
