Cat. #152848
BICR 6 Cell Line
Cat. #: 152848
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Hypopharynx
Disease: Cancer
Model: Tumourigenic cell line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Eric Kenneth Parkinson
Institute: Cancer Research UK Glasgow: The Beatson Institute
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: BICR 6 Cell Line
- Alternate name: bicr6; BICR-6; BICR6; Beatson Institute for Cancer Research 6; HNSCC; SCC-HN
- Cancer: Head and neck cancer
- Cancers detailed: Hypopharynx squamous cell carcinoma
- Research fields: Apoptosis and autophagy;Cancer;Drug development;Genetics
- Organism: Human
- Tissue: Hypopharynx
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumourigenic cell line
- Conditional: Yes
- Description: BICR 6 is an adherent keratinocyte cell line derived from a squamous cell carcinoma of the hypopharynx of a Caucasian male. The tumour stage (TNM) of the culture is T4 N1 M0. Keratin and involucrin markers are present. Epidermal Growth Factor Receptor (EGFR) is also present. Known mutations: p53, p16 and p14ARF. These cells are tumourigenic in athymic mice.
- Additional notes: STR-PCR Data: Amelogenin: X, Y CSF1PO: 10 D13S317: 14 D16S539: 11 D5S818: 12 D7S820: 10 THO1: 6 TPOX: 9,11 vWA: 16,17
- Cellosaurus id: CVCL_2314
Applications
- Application notes: STR-PCR Data: Amelogenin: X, Y CSF1PO: 10 D13S317: 14 D16S539: 11 D5S818: 12 D7S820: 10 THO1: 6 TPOX: 9,11 vWA: 16,17
Handling
- Format: Frozen
- Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 µg/ml Hydrocortisone.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Split sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.05% trypsin/EDTA; 8% CO₂; 37°C. Suggested seeding density of 2 x 10,000 cells/cm². Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells. Feeder layers are prepared in the flasks at least 24 hours in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in a 37°C water bath and the contents quickly transferred to a 15ml centrifuge tube. DMEM medium is added drop wise to 5ml. Cells are centrifuged at 150 x g for 5 minutes at Room Temperature. Cells are resuspended in 5ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 10^4 cells/cm².
Related Tools
- Related tools: BICR 10 Cell Line ; BICR 16 Cell Line ; BICR 18 Cell Line ; BICR 22 Cell Line ; BICR 3 Cell Line ; BICR 31 Cell Line ; BICR 56 Cell Line ; BICR 78 Cell Line ; BICR 82 Cell Line
References
- Oh et al. 2021. Nat Commun. 12(1). PMID: 34389714.
- Leonard et al. 2019. Oral Oncol. 95:35-42. PMID: 31345392.
- Agochiya et al. 1999. Oncogene. 18(41):5646-5653. PMID: 10523844.
- Edington et al. 1995. Mol Carcinog. 13(4):254-265. PMID: 7646764.
