BICR 22 Cell Line

Adherent cell line derived from a lymph node metastasis squamous cell carcinoma of the tongue of a Caucasian male. Keratin and involucrin markers present.

Contributors

Inventor Institute
Eric Kenneth Parkinson Cancer Research UK Glasgow: The Beatson Institute
Cat. #: 152844
Unit size: 1×10^6 cells / vial
Research Fields: Apoptosis and autophagy;Cancer;Drug development;Genetics
Organism: Human
Tissue: Tongue
Model: Tumourigenic cell line
Growth properties: Adherent
Cancer Types In Detail: Tongue squamous carcinoma (lymph node metastasis)
Alternate name: bicr22; BICR-22; BICR22; Beatson Institute for Cancer Research 22; HNSCC; SCC-HN
Product description: Adherent cell line derived from a lymph node metastasis squamous cell carcinoma of the tongue of a Caucasian male. Keratin and involucrin markers present.
Conditional: Yes
Additional notes: Amelogenin: X, X CSF1PO: 11 D13S317: 11,13 D16S539: 11,12 D5S818: 11,12 D7S820: 11,12 THO1: 6,7 TPOX: 8 vWA: 16,19
Cellosaurus ID: CVCL_2310
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 2mM Glutamine + 2% Foetal Bovine Serum (FBS) + 0.4 µg/ml Hydrocortisone.
Subculture routine: Split sub-confluent cultures (70-80%) 1:10 – 1:50 using 0.05% trypsin/EDTA; 8% CO₂; 37°C. Suggested seeding density 2 x 10,000 cells/cm². Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells (ECACC Catalogue number: 85022108). Feeder layers are prepared in the flasks at least 24 hours in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in 37°C water bath and the contents quickly transferred to a 15ml centrifuge tube. DMEM medium is added drop wise to 5ml. Cells are centrifuged at 150 x g for 5 minutes at Room Temperature. Cells are resuspended in 5ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 10^4 cells/cm².
References:

Loughran et al. 1997. Oncogene. 14(16):1955-1964. PMID: 9150362.

Edington et al. 1995. Mol Carcinog. 13(4):254-265. PMID: 7646764.

Images

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BICR 22 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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