#151133

Anti-Integrin b3 [MHF4]

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Cat. #151133

Anti-Integrin b3 [MHF4]

Cat. #: 151133

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Integrin ?3 chain (CD61)

Class: Monoclonal

Application: FACS ; IHC ; FACS ; IHC ; IF ; IP

Reactivity: Human ; Rat

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Mike Horton

Institute: Queen Mary University of London

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-Integrin b3 [MHF4]
  • Research fields: Cell biology;Tissue-specific biology;Cell signaling and signal transduction
  • Clone: MHF4
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 118/100
  • Strain: Balb/c
  • Reactivity: Human ; Rat
  • Host: Mouse
  • Application: FACS ; IHC ; FACS ; IHC ; IF ; IP
  • Description: The MHF4 epitope is different to the F11 epitope.
  • Immunogen: A bone cell suspension containing osteoclasts, osteoblasts and bone marrow cells obtained from the long bones of newborn Sprague Dawley rats.
  • Isotype: IgG1
  • Myeloma used: P3X63Ag8.653
  • Recommended controls: The antigen is found in granule components of mast cells.

Target Details

  • Target: Integrin ?3 chain (CD61)
  • Molecular weight: 118/100
  • Target background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin b3 is expressed in osteoclasts, megacaryocytes and platelets. MHF4 may be used to identify osteoclasts in tissue sections and bone cell suspensions.

Applications

  • Application: FACS ; IHC ; FACS ; IHC ; IF ; IP

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Human Adenovirus Type 37 Uses aV?1 and a3?1 Integrins for Infection of Human Corneal Cells.
  • Storm et al. 2016. J Virol. :. PMID: 27974569.
  • Ballana et al. 2011. J Immunol. 186(1):464-70. PMID: 21098231.
  • ?5 integrin is the major contributor to the aVintegrin-mediated blockade of HIV-1 replication.
  • Helfrich et al. 1992. J Bone Miner Res. 7(3):345-51. PMID: 1585837.
  • Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3.