Cat. #153288
phOC-iNOS Vector
Cat. #: 153288
Sub-type: pcDNA 3.1
Availability: 3-5 days
Target: Inducible Nitric Oxide Synthase
Bacterial Resistance: Kanamycin
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Helen McCarthy
Institute: Queen's University Belfast
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: phOC-iNOS Vector
- Alternate name: Nitric Oxide Synthase
- Research fields: Cancer;Metabolism;Neurobiology
- Tool sub type: pcDNA 3.1
- Bacterial resistance: Kanamycin
- Description: phOC-iNOS Plasmid is a human nitric oxide synthase (iNOS) transgene driven by a human osteocalcin (hOC) promoter to control inducible nitric oxide synthase (iNOS) transgene expression in hormone refractory prostate cancer (HRPC). The plasmid was originally developed as an anti-cancer therapeutic with a view to a gene therapy strategies. Studies in the literature have demonstrated that this plasmid increases iNOS protein and total nitrite levels in PC3 and DU145 cells, but not LNCaP or HT29 (McCarthy et al., 2007). The plasmid serves as a useful tool in cardiovascular studies or any physiology associated with vasodilation.
Handling
- Storage conditions: -80° C
- Shipping conditions: Dry Ice
Target Details
- Target: Inducible Nitric Oxide Synthase
Application Details
- Application notes: Recommended for transpormation in DH5 alpha bacterial cells iNOS gene is 4.4kb
Related Tools
- Related tools: pCMV-iNOS Vector
References
- McCarthy et al. 2007. J Gene Med. 9(6):511-20. PMID: 17471586.
- Human osteocalcin: a strong promoter for nitric oxide synthase gene therapy, with specificity for hormone refractory prostate cancer.
