#153456

pGEX4T1-CBL-B Vector

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Cat. #153456

pGEX4T1-CBL-B Vector

Cat. #: 153456

Sub-type: pGEX4T1

Availability: 3-5 days

Target: CBL-B fragment encompassing the TKBD, LHR and RING domain: residues 36–427

Bacterial Resistance: Ampicillin

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Danny Huang

Institute: Cancer Research UK, Glasgow: The Beatson Institute

Tool Details
Handling
Target Details
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: pGEX4T1-CBL-B Vector
  • Alternate name: C-CBL, CBL-B, CBL-C, C-CBL2, FRA11B, NSLL, RNF55, Cbl proto-oncogene, Casitas B-lineage Lymphoma
  • Research fields: Cancer;Cell signaling and signal transduction;Genetics
  • Tool sub type: pGEX4T1
  • Backbone size without insert: 4969
  • Bacterial resistance: Ampicillin
  • Description: Over 600 E3 ligases have been identified in mammals and most are part of the RING family of E3 ligases. The monomeric family of CBL RING E3 ligases (c-CBL, CBL-B and CBL-C) attenuate nonreceptor and receptor tyrosine kinase signalling through ubiquitination and direct the receptor tyrosine kinases for degradation through the endocytic or proteasomal pathways. Members of the CBL family share a highly conserved N terminus comprising of a tyrosine kinase binding domain, a linker helix region and a RING domain. The more variable C terminus possessing a proline-rich region and a string of terminal amino acids or 'extension'. Mutations in this gene have been implicated in several human cancers including acute myeloid leukaemia. The protein CBL is a ubiquitously expressed 982 amino acid E3 ubiquitin-protein ligase involved in cell signalling and protein ubiquitination.

Handling

  • Storage conditions: -20° C
  • Shipping conditions: Dry Ice

Target Details

  • Target: CBL-B fragment encompassing the TKBD, LHR and RING domain: residues 36–427

Related Tools

  • Related tools: pRSF-1b-UbcH5B Vector

References

  • Dou et al. 2013. Nat Struct Mol Biol. 20(8):982-6. PMID: 23851457.
  • Essentiality of a non-RING element in priming donor ubiquitin for catalysis by a monomeric E3.