Cat. #162284

pBABE-hygro Vector

Name: pBABE-hygro Vector
SKU: 162284
Availability: InStock

Cat. #: 162284

Unit size: Provided as 50-100ng cloning grade DNA on Whatman filter paper

Bacterial Resistance: Ampicillin

Selectable Markers: Hygromycin

£65.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

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Contributor

Inventor: Hartmut Land, Jay Morgenstern

Institute: Cancer Research UK London Research Institute: Lincoln's Inn Fields

Primary Citation: Morgenstern et al. 1990. Nucleic Acids Res. 18(12):3587-96. PMID: 2194165.

Tool Details

Handling

References

Documentation

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

Tool name: pBABE-hygro Vector
Research fields: Genetics
Total vector size: 5558
Backbone size without insert: 5558
Bacterial resistance: Ampicillin
Selectable markers: Hygromycin
Description: High-titer retroviral empty backbone pBABE vector, ready for cloning. pBABE-hygro enables stable gene delivery and expression in mammalian cells with MO-MuLV LTR-driven expression for reliable, long-term transgene production. Features Hygromycin B selection useful for sequential selection studies. pBABE-hygro is a part of the established pBABE vector system, developed by Morgenstern and Land 1990. Key features include: high viral titers for efficient transduction, stable long-term gene expression via LTR promoter and helper-free design which minimises contamination risk.
Additional notes: The pBABE vectors prove useful in experiments where highest frequencies of gene transfer, or concomitant expression of several different genes within a single cell are required with minimal risk of helper virus contamination.

Handling

Shipping conditions: Provided as 50-100ng cloning grade DNA on Whatman filter paper
Plasmid amplification details: DH5alpha 37° C
Plasmid copy number: High copy

References

Morgenstern et al. 1990. Nucleic Acids Res. 18(12):3587-96. PMID: 2194165. Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.

Documentation

Full pBABE Hygro DNA sequence .DNA