Anti-ICAM1 [15.2]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-ICAM1 [15.2]
• Alternate name: Intercellular Adhesion Molecule 1; Major Group Rhinovirus Receptor; ICAM-1; Cell Surface Glycoprotein P3.58; Human Rhinovirus Receptor; P3.58; CD54; BB2
• Research fields: Cell biology;Immunology;Stem cell biology;Tissue-specific biology
• Clone: 15.2
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 85-115 kDa
• Strain: Balb/c
• Reactivity: Human
• Host: Mouse
• Application: FACS ; IHC ; IP ; Fn
• Description: ICAMs are members of the immunoglobulin superfamily that is characterised by the presence of immunoglobulin-like domains. ICAM-1 is expressed in haemopoietic cells and vascular endothelium. Cytokine activation causes ICAM-1 expression in other cell types such as fibroblasts and keratinocytes. ICAM-1 is involved in leukocyte recruitment and inflammation. ICAM-1 binds LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18).
• Immunogen: Rheumatoid synovial cells and human monocytes.
• Isotype: IgG1
• Myeloma used: Sp2/0-Ag14
• Recommended controls: Tonsil

Target Details

• Target: ICAM1 (CD54)
• Molecular weight: 85-115 kDa
• Tissue cell line specificity: Tonsil
• Target background: ICAMs are members of the immunoglobulin superfamily that is characterised by the presence of immunoglobulin-like domains. ICAM-1 is expressed in haemopoietic cells and vascular endothelium. Cytokine activation causes ICAM-1 expression in other cell types such as fibroblasts and keratinocytes. ICAM-1 is involved in leukocyte recruitment and inflammation. ICAM-1 binds LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18).

Applications

• Application: FACS ; IHC ; IP ; Fn

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -20° C
• Shipping conditions: Dry ice

References

• Vsquez et al. 2016. Microbes Infect. :. PMID: 27717894.
• Plasmodium falciparum isolates from patients with uncomplicated malaria promote endothelial inflammation.
• Intercellular adhesion molecule 1 serves as a primary cognate receptor for the Type IV pilus of nontypeable Haemophilus influenzae.
• Novotny et al. 2016. Cell Microbiol. :. PMID: 26857242.
• Perturbation of adhesion molecule-mediated chondrocyte-matrix interactions by 4-hydroxynonenal binding: implication in osteoarthritis pathogenesis.
• El-Bikai et al. 2010. Arthritis Res Ther. 12(5):R201. PMID: 20977750.
• Conway et al. 2010. Am J Physiol Heart Circ Physiol. 298(2):H367-74. PMID: 19915176.
• Endothelial cell responses to atheroprone flow are driven by two separate flow components: low time-average shear stress and fluid flow reversal.
• Lauriello et al. 2005. Acta Otorhinolaryngol Ital. 25(5):284-91. PMID: 16602327.
• A two-year course of specific immunotherapy or of continuous antihistamine treatment reverse eosinophilic inflammation in severe persistent allergic rhinitis.
• Stanley et al. 2000. Biochem J. 351(Pt 1):79-86. PMID: 10998349.
• The second domain of intercellular adhesion molecule-1 (ICAM-1) maintains the structural integrity of the leucocyte function-associated antigen-1 (LFA-1) ligand-binding site in the first domain.
• Dransfield et al. 1992. J Cell Biol. 116(6):1527-35. PMID: 1541641.
• Interaction of leukocyte integrins with ligand is necessary but not sufficient for function.