#151042

Anti-LewisX [29]

Cat. #151042

Anti-LewisX [29]

Cat. #: 151042

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: Lewis X (CD15)

Class: Monoclonal

Application: FACS

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Nancy Hogg

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-LewisX [29]
  • Alternate name: Fucosyltransferase 4; Galactoside 3-L-Fucosyltransferase; ELAM-1 Ligand Fucosyltransferase; FUC-TIV; FCT3A; ELFT; Stage-Specific Embryonic Antigen; Alpha (1,3) Fucosyltransferase; EC 2.4.1.65; Lewis X; SSEA-1; FUTIV; CD15; LeX
  • Research fields: Cancer;Immunology
  • Clone: 29
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 83 kDa
  • Strain: Balb/c
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS
  • Description: Monoclonal antibody directed at against Lewis X antigen, with use in differentiating between leukaemia's and aids in the purification of stem cells.
  • Immunogen: Fibronectin purified human monocytes .
  • Immunogen uniprot id: P22083
  • Isotype: IgM
  • Myeloma used: Sp2/0-Ag14

Target Details

  • Target: Lewis X (CD15)
  • Molecular weight: 83 kDa
  • Target background: Lewis X (CD15) is a branched pentasaccharide found on glycolipids, glycoproteins and proteoglycans of cells of gastric mucosa, nervous system, neutrophils, eosinophils, and monocytes. Lewis X is distributed abnormally in myeloid leukaemia's and is commonly used in the diagnosis of Hodgkin's disease. Lewis X is believed to enhance cell adhesion, either directly or by promoting integrin activation. Cells with high surface expression of Lewis X antigen, display strong self-aggregation, based on calcium-dependent Lewis X-Lewis X interaction. Anti-Lewis X (29) is useful for leukaemia typing and the purification of stem cells.

Applications

  • Application: FACS

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C

References

  • Bernstein I & Self S. Report of the myeloid section of the Second International Workshop on Leucocyte Typing II, Human leucocyte Differentiation Antigens. Published by Springer-Verlag, New York. 1986.