pBABE-zeo Vector

High-titer retroviral empty backbone pBABE vector, ready for cloning. pBABE-zeo enables stable gene delivery and expression in mammalian cells with MO-MuLV LTR-driven expression for reliable, long-term transgene production. Features Zeocin selection – a potent selection marker. pBABE-zeo is a part of the established pBABE vector system, developed by Morgenstern and Land 1990. Key features include: […]

Contributors

Inventor Institute
Hartmut Land, Jay Morgenstern Cancer Research UK London Research Institute: Lincoln's Inn Fields
Cat. #: 162285
Tool sub type: 10XUAS IVS p10
Unit size: Provided as 50-100ng cloning grade DNA on Whatman filter paper
Research Fields: Genetics;Genetics
Total vector size: 4888
Backbone size without insert: 4888
Bacterial resistance: Ampicillin
Selectable markers: Zeocin
Primary citation: Morgenstern et al. 1990. Nucleic Acids Res. 18(12):3587-96. PMID: 2194165.
Product description: High-titer retroviral empty backbone pBABE vector, ready for cloning. pBABE-zeo enables stable gene delivery and expression in mammalian cells with Mo-MuLV LTR-driven expression for reliable, long-term transgene production. Features Zeocin selection – a potent selection marker. pBABE-zeo is a part of the established pBABE vector system, developed by Morgenstern and Land 1990. Key features include: high viral titers for efficient transduction, stable long-term gene expression via LTR promoter and helper-free design which minimises contamination risk.
Additional notes: The pBABE vectors prove useful in experiments where highest frequencies of gene transfer, or concomitant expression of several different genes within a single cell are required with minimal risk of helper virus contamination.
Shipping conditions: Provided as 50-100ng cloning grade DNA on Whatman filter paper
Plasmid amplification details: DH5alpha 37° C
Plasmid copy number: High copy

Citations And Reviews

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