pBABE-puro Vector

pBABE-puro empty backbone vector by Morgenstern and Land. Puromycin selection, high-titer retroviral gene delivery. Ready for custom gene cloning.

Contributors

Inventor Institute
Hartmut Land, Jay Morgenstern Cancer Research UK London Research Institute: Lincoln's Inn Fields
Cat. #: 151470
Tool sub type: 10XUAS IVS p10
Unit size: Provided as 50-100ng cloning grade DNA on Whatman filter paper
Research Fields: Genetics;Genetics
Total vector size: 5169
Backbone size without insert: 5169
Bacterial resistance: Ampicillin
Selectable markers: Puromycin
Primary citation: Morgenstern et al. 1990. Nucleic Acids Res. 18(12):3587-96. PMID: 2194165.
Product description: High-titer retroviral empty backbone pBABE vector, ready for cloning. pBABE-puro enables stable gene delivery and expression in mammalian cells with MO-MuLV LTR-driven expression for reliable, long-term transgene production. Features puromycin selection for rapid cell selection and broad cell line applicability. pBABE-puro is a part of the established pBABE vector system, developed by Morgenstern and Land 1990. Key features include: high viral titers for efficient transduction, stable long-term gene expression via LTR promoter and helper-free design which minimises contamination risk.
Additional notes: The pBABE vectors prove useful in experiments where highest frequencies of gene transfer, or concomitant expression of several different genes within a single cell are required with minimal risk of helper virus contamination.
Shipping conditions: Provided as 50-100ng cloning grade DNA on Whatman filter paper
Plasmid amplification details: DH5alpha 37° C
Plasmid copy number: High copy
References:

Morgenstern et al. 1990. Nucleic Acids Res. 18(12):3587-96. PMID: 2194165.

Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.

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