GST-tagged human cannabinoid CB1 receptor (amino acids 414 to 442)

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Tool name: GST-tagged human cannabinoid CB1 receptor (amino acids 414 to 442)
• Research fields: Biochemistry;Cell biology;Molecular Biology;Neurobiology
• Tool sub type: GST fusion protein
• Sequence: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
• Description: Polypeptide spanning amino acids 414 to 442 (CB1414–442) of the cytosolic tail of the human cannaboinoid CB1 receptor fused to the C-terminus of the glutathione S-transferase (GST) tag. The linker between the GST tag and the CB1414–442 polyopeptide posses the consensus cleavage site LeuPheGlnGlyPro for the PreScission protease for removing the GST tag after purification.
• Application: Negative control for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides within the sequence spanning amino acids 443-472 at the C-terminus of the CB1 receptor. Negative control in protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 of the C-terminal end of the CB1 receptor.
• Tag: Glutathione S-transferase (GST)
• Molecular weight: 29710.86 Da
• Expression system: Escherichia coli
• Additional notes: Contact the inventor for further information.

Handling

• Format: Solution
• Purity: Affinity purified
• Storage conditions: -80° C
• Storage buffer: 126 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM EDTA, pH 7.4Contains 50 mM reduced glutathione which may need to be removed (e.g. by dialysis) for certain assays.
• Shipping conditions: Frozen in dry ice

Application Details

• Application: Negative control for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides within the sequence spanning amino acids 443-472 at the C-terminus of the CB1 receptor. Negative control in protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 of the C-terminal end of the CB1 receptor.
• Application notes: Negative control for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides within the sequence spanning amino acids 443-472 at the C-terminus of the CB1 receptor. Negative control in protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 of the C-terminal end of the CB1 receptor.

References

• Saumell-Esnaola et al. 2022 Microb Cell Fact.21(1):192. PMID: 36109736
• Gómez-Caballero et al. 2021. Mikrochim Acta. 188(11):368. PMID: 34618242.