Cat. #162173
Nrf3 deficient mouse
Cat. #: 162173
Sub-type: Mouse
Availability: 3-4 weeks
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Volker Blank
Institute: McGill University
Primary Citation: Blank V. et al. 2004. Molecular and Cellular Biology. Apr. v24(8). PMID: 15060151
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: Nrf3 deficient mouse
- Research fields: Cancer
- Tool sub type: Mouse
- Description: The model was created by replacing two exons of the Nfe2l3 gene, comprising sequences coding for the bZIP domain of the transcription factor by neomycin-cytosine deaminase cassette of the targeting vector. 129S6 ES cells were selected by resistance to G418. The targeted cells were injected into C57BL/6J blastocysts. Resultant chimeras were backcrossed for more than 10 generations. Neomycin-cytosine cassette flanked by loxP sites has been removed by breeding to Cre mice (C57BL/6-Tg(Zp3-cre)93Knw/J). Knockout mice have been generated in 129S6, C57BL/6 and FVB backgrounds.
- Phenotype: Nfe2l3-/- mice develp normallyand are fertile.
- Zygosity: Homozygous
- Strain: Knockout mice have been generated in the following backgrounds: 129S6, C57BL/6, FVB
- Production details: The model was created by replacing two exons of the Nfe2l3 gene, comprising sequences coding for the bZIP domain of the transcription factor by neomycin-cytosine deaminase cassette of the targeting vector. 129S6 ES cells were selected by resistance to G418. The targeted cells were injected into C57BL/6J blastocysts. Resultant chimeras were backcrossed for more than 10 generations. Neomycin-cytosine cassette flanked by loxP sites has been removed by breeding to Cre mice (C57BL/6-Tg(Zp3-cre)93Knw/J). Knockout mice have been generated in 129S6, C57BL/6 and FVB backgrounds.
Handling
- Shipping conditions: Sperm / dry ice
References
- Blank V. et al. 2004. Molecular and Cellular Biology. Apr. v24(8). PMID: 15060151
