MAGL Knockout mouse

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Tool name: MAGL Knockout mouse
• Alternate name: MGL, Mgll, Magl
• Research fields: Cell signaling and signal transduction;Metabolism
• Tool sub type: Mouse
• Model: Knock-Out
• Conditional: No
• Description: Useful model to study MAGL-deficiency. Animals might be useful for studies, e.g. on energy metabolism or endocannabinoid signalling.
• Genetic background: These mice were generated using HM1-ES cells. ES cell clones were injected into C57BL/6J blastocysts to enable coat colour selection of the chimeras. Mice were subsequently backcrossed to C57BL/6J (≥ 10 generations). When kept as a homozygous colony, mice are backcrossed regularly to C57BL/6J to avoid generation of subpopulations.
• Phenotype: These mice display a mildly attenuated peripheral lipolysis, and are resistant to diet-induced insulin resistance and hepatic steatosis. Further, these mice display a desensitization of intestinal cannabinoid receptor type 1, which leads to accelerated colonic propulsion.
• Zygosity: Homozygous
• Strain: C57BL/6J
• Production details: A floxed neomycin resistance cassette was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 4. Cre-mediated recombination removed Mgll exon 3 and 4 and the selection cassette. Further details are available upon request
• Breeding information: Good breeder. Homozygous mice may be bred together to maintain a live colony.

Target Details

• Target: Monoglyceride lipase; monoacyl glycerol lipase

Related Tools

• Related tools: MAGL-Flox mouse

References

• 26075589
• Taschler et al. 2011. J Biol Chem. 286(20):17467-77. PMID: 21454566.